In this paper, we describe multivalent display of peptide and protein sequences typically censored from traditional N-terminal display on protein pIII of filamentous bacteriophage M13. Using site-directed mutagenesis of commercially available M13KE phage cloning vector, we introduced sites that permit efficient cloning using restriction enzymes between domains N1 and N2 of the pIII protein. As ...

Messing J, Crea R & Seeburg P H. A system for shotgun DNA sequencing. Nucl. Acid. Res. 9:309-21, 1981. [Dept. Bacteriology, Univ. California, Davis, CA] Messing J & Vieira J. A new pair of M13 vectors for selecting either DNA strand of double digest restriction fragments. Gene 19:269-76, 1982. [Dept. Biochemistry, Univ. Minnesota, St. Paul, MN] Yanisch-Perron C, Vieira J & Messing J. Improved M...

Aqueous two-phase systems allow for the unequal distribution of proteins and other molecules in water-rich solutions containing phase separating polymers or surfactants. One approach to improve the partitioning properties of recombinant proteins is to produce the proteins as fused to certain peptide tags. However, the rational design of such tags has proven difficult since it involves a comprom...

We assembled single-stranded DNA (ssDNA) conjugated nanoparticles using the phage M13 gene 5 protein (g5p) as the molecular glue to bind two antiparallel noncomplementary ssDNA strands. The entire process was controlled tightly by the concentration of the g5p protein and the presence of double-stranded DNA. The g5p-ssDNA aggregate was disintegrated by hybridization with complementary ssDNA (C-s...

Source/Description: A phage library was constructed in dephosphorylated BamHI digested M13 from size-selected (220±1000 bp) turkey genomic DNA restricted with MboI. The library was screened with P-labeled (CA)16 and (GT)16 probes. Positive clones were sequenced with an automated DNA sequencer (ABI 373) at the Advanced Genetic Analysis Center, College of Veterinary Medicine, University of Minnes...

Amphiphilic macromolecules containing a polystyrene-adherent peptide domain and a cell-repellent poly(ethylene glycol) domain were designed, synthesized, and evaluated as a cytophobic surface coating. Such cytophobic, or cell-repellent, coatings are of interest for varied medical and biotechnological applications. The composition of the polystyrene binding peptide domain was identified using an...

Inter- and intraspecies horizontal gene transfer enabled by bacterial secretion systems is a powerful mechanism for bacterial genome plasticity. The type IV secretion system of Escherichia coli, encoded by the F plasmid, enables cell-to-cell contact and subsequent DNA transfer known as conjugation. Conjugation is compromised by phage infection that specifically targets the secretion machinery. ...