Background MIF via its receptor, CD74, initiates a signaling cascade that leads to proliferation and survival of cells. Also, MIF binding to CD74 activates p38 signaling pathways that lead to positive effect on the expression of COX-2. The aim of this study was to evaluate the gene expression profile of MIF, CD74 and COX-2 in normal, ectopic and eutopic endometrium during menstrual cycle. The e...

The capillary tube technique for assaying macrophage migration (1) has been widely used in studies on cellular immunity and has allowed a better understanding of lymphocyte-macrophage interaction associated with the expression of delayed hypersensitivity. The sensitive lymphocyte on exposure to specific antigen (2, 3) has been shown to produce a soluble effector molecule called migration inhibi...

The plasma esterase inhibitors alpha2-macroglobulin, alpha1-antitrypsin, C1-inhibitor, antithrombin-heparin cofactor, and, as previously described, soybean trypsin inhibitor (Kunitz) and diisopropylphosphorofluoridate (9) enhance the response of guinea pig macrophages to migration inhibitory factor. To obtain this effect, macrophages are incubated with inhibitors prior to assay. The data sugges...

Macrophage migration inhibitory factor (MIF) is a pleiotropic protein with catalytic, CD74, CXCR2, CXCR4, and nuclease activities that contribute to the pathology of various inflammatory disorders, cardiovascular diseases, cancer. The majority MIF-triggered pathological conditions are associated activation MIF’s cell surface receptor. mechanistic details MIF-induced CD74 were mostly unknown unt...

Soluble membrane fractions derived from polyoma tumor cells trigger lymphocytes, derived from polyoma-immunized animals, but not from nonimmunized controls, to release the lymphokine, macrophage migration-inhibitory factor. The reaction can be blocked by sera from polyoma-bearing animals. Absorption of these sera with polyoma cells, but not with nonpolyoma cell lines, abrogates this activity. T...