نتایج جستجو برای: maldi tof mass spectrometry
تعداد نتایج: 492446 فیلتر نتایج به سال:
Accurate species identification of the Sporothrix schenckii complex is essential, since identification based only on phenotypic characteristics is often inconclusive due to phenotypic variability within the species. We used matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for species identification of 70 environmental and clinical isolates of the Sporo...
Current diagnostic and therapeutic strategies for lung cancer have had no significant impact on lung cancer mortality over the last several decades. This study used a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) discovery platform to generate protein expression profiles in search of overexpressed proteins in lung tumors as potentially novel molecul...
Technological advances have changed the practice of clinical microbiology. We implemented Bruker matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and BD Kiestra total laboratory automation (TLA) 4 and 3 years ago, respectively. To assess the impact of these new technologies, we compared turnaround times (TATs) for positive and negative urine cultures b...
A facile method for accurate detection of type 1 dengue virus (DV1) infection from complex biological mixtures, using type specific immunocapture coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), was developed. The biological mixtures were treated with magnetic particles coated with a monoclonal antibody directly against type 1 dengue viru...
The term “proteome” refers to the total of all proteins expressed in an organism. “proteomics” field research that includes not only information on expression levels individual proteins, but also their higher-order structures, intermolecular interactions, and post-translational modifications. core technology, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), is available...
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), which provides high sensitivity and resolution, has been used for the analysis of biopolymers. In past decade, MALDI-TOF MS utilized in clinical fields around world to identify microbial species. However, serotype or strain discrimination same bacterial species is difficult by MS. Different methods such...
Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometers are simple and robust mass spectrometers used for analysis of biologically relevant molecules in diverse fields including pathogen identification, imaging mass spectrometry, and natural products chemistry. Despite high nominal resolution and accuracy, we have observed significant variability where 30-50% o...
Abbreviations: ACN: Acetonitrile; AvBD: Avian Beta-Defensin; CAM: Carbamidomethylation; 1,5 DAN: 1,5 Diaminonaphthalene; DHB: 2,5 Dihydroxybenzoicacid; DTT: Dithiothreitol; FITC: Fluorescein Isothiocyanate; IAA: Iodoacetamide; ISD: In Source Decay; MALDI-MS: Matrix-Assisted Laser Desorption Ionization Mass Spectrometry; m/z: Mass/Charge; PI: Propidiumiodide; RP-HPLC: Reverse Phase-High Pressure...
The certification of a polystyrene standard reference material, SRM 2888, is described. The Mw of SRM 2888 was determined by light scattering to be 7.19x10 g/mol with a sample standard deviation of 0.14 x10 g/mol. A combined expanded uncertainty for this light scattering Mw determination, including systematic and random uncertainties, was estimated to be 0.57 x10 g/mol. Mn was determined by nuc...
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