Classical approaches for analyzing reverse transcription quantitative polymerase chain reaction (RT-qPCR) data commonly require normalization before assessing differential expression (DE). Normalization often has a substantial effect on the interpretation and validity of the subsequent analysis steps, but at the same time it causes a reduction in variance and introduces dependence among the nor...

The authors give the exact coefficient of 1/N in a saddlepoint approximation to the Wilcoxon-Mann-Whitney null-distribution. This saddlepoint approximation is obtained from an Edgeworth approximation to the exponentially tilted distribution. Moreover, the rate of convergence of the relative error is uniformly of order O(1/N) in a large deviation interval as defined in Feller (1971). The propose...

Most conventional statistical tests for analyzing RT-qPCR data require normalization before differential expression can be assessed. This normalization can have a substantial effect on the interpretation and validity of the statistical test, but this effect is often ignored. Therefore the uWMW test, as proposed in [1], extends the Wilcoxon–Mann–Whitney test so that the normalization is incorpor...

A program for neuronal activity analysis is described. The applied computational techniques are standard in the field of digital processing, but the program is particular in its field of application and mode of data selection. The program, written in Turbo Pascal for the IBM-PC and compatibles, statistically compares (Mann-Whitney U-test) two sets of graphically selected data, and establishes w...

Common complex diseases are likely influenced by the interplay of hundreds, or even thousands, of genetic variants. Converging evidence shows that genetic variants with low marginal effects (LMEs) play an important role in disease development. Despite their potential significance, discovering LME genetic variants and assessing their joint association on high-dimensional data (e.g., genome-wide ...

MOTIVATION Recently, De Neve et al. proposed a modification of the Wilcoxon-Mann-Whitney (WMW) test for assessing differential expression based on RT-qPCR data. Their test, referred to as the unified WMW (uWMW) test, incorporates a robust and intuitive normalization and quantifies the probability that the expression from one treatment group exceeds the expression from another treatment group. H...

An accurate and early diagnosis of the Alzheimer’s Disease (AD) is of fundamental importance for the development of effective treatments to palliate the effects of the disease. Computer Aided Diagnosis (CAD) allows physicians to detect early stages of the disease, and functional brain images have been proved to be very useful in this task. This paper presents a new CAD system that consists of t...

The Pemberton Happiness Index (PHI) is a recently developed integrative measure of well-being that includes components of hedonic, eudaimonic, social, and experienced well-being. The PHI has been validated in several languages, but not in Portuguese. Our aim was to cross-culturally adapt the Universal Portuguese version of the PHI and to assess its psychometric properties in a sample of the Bra...

BACKGROUND Subtypes of glioblastoma multiforme (GBM) based on genetic and molecular alterations are thought to cause alterations in anatomic MRI owing to downstream biological changes, such as edema production, blood-brain barrier breakdown, and necrosis. The purpose of the current study was to identify a potential relationship between imaging features and the mesenchymal (MES) GBM subtype, whi...