نتایج جستجو برای: mannose binding protein mbp

تعداد نتایج: 1452875  

Journal: :The Journal of Experimental Medicine 1978
R S Fujinami P Y Paterson E D Day V A Varitek

Age-related concentrations of myelin basic protein serum factor (MBP-SF), an endogenous neuroantigen detected and quantitated by inhibition of binding of rat myelin basic protein (RMBP) antibody with 125I-RMBP reagent antigen and immunochemically indistinguishable from native RMBP in this respect, reach peak levels as high as 21 ng/microliter among 2-3-wk-old normal suckling Lewis rats. Levels ...

Journal: :The EMBO journal 2011
Yiyuan Yin Yili Li Melissa C Kerzic Roland Martin Roy A Mariuzza

The failure to eliminate self-reactive T cells during negative selection is a prerequisite for autoimmunity. To escape deletion, autoreactive T-cell receptors (TCRs) may form unstable complexes with self-peptide-MHC by adopting suboptimal binding topologies compared with anti-microbial TCRs. Alternatively, escape can occur by weak binding between self-peptides and MHC. We determined the structu...

2015
Luthur Siu-Lun Cheung Daniel J. Shea Nathan Nicholes Amol Date Marc Ostermeier Konstantinos Konstantopoulos

Monobodies are antibody alternatives derived from fibronectin that are thermodynamically stable, small in size, and can be produced in bacterial systems. Monobodies have been engineered to bind a wide variety of target proteins with high affinity and specificity. Using alanine-scanning mutagenesis simulations, we identified two scaffold residues that are critical to the binding interaction betw...

Journal: :Journal of biochemistry 2000
H Furukawa T Haga

The M2 muscarinic acetylcholine receptor mutant (M2 mutant), with a lack of glycosylation sites, a deletion in the central part of the third inner loop, and the addition of a six histidine tag at the C-terminus, was fused to maltose binding protein (MBP) at its N-terminus and expressed in Escherichia coli. The expression level was 0.2 nmol receptor per 100 ml culture, as assessed as [3H]L-quinu...

2015
Zhen Gong Jose M. Martin-Garcia Sasha M. Daskalova Felicia M. Craciunescu Lusheng Song Katerina Dörner Debra T. Hansen Jay-How Yang Joshua LaBaer Brenda G. Hogue Tsafrir S. Mor Petra Fromme Anil Kumar

The membrane proximal region (MPR, residues 649-683) and transmembrane domain (TMD, residues 684-705) of the gp41 subunit of HIV-1's envelope protein are highly conserved and are important in viral mucosal transmission, virus attachment and membrane fusion with target cells. Several structures of the trimeric membrane proximal external region (residues 662-683) of MPR have been reported at the ...

Journal: :The Journal of neuroscience : the official journal of the Society for Neuroscience 2000
Z Li Y Zhang D Li Y Feng

Quakingviable (qk(v)) is a well known dysmyelination mutation. Recently, the genetic lesion of qk(v) has been defined as a deletion 5' to the qkI gene, which results in the severe reduction of the qkI-encoded QKI RNA-binding proteins in myelin-producing cells. However, no comprehensive model has been proposed regarding how the lack of QKI leads to dysmyelination. We hypothesized that QKI binds ...

Journal: :Journal of bacteriology 1997
G Merino H A Shuman

Escherichia coli accumulates malto-oligosaccharides by the maltose transport system, which is a member of the ATP-binding-cassette (ABC) superfamily of transport systems. The proteins of this system are LamB in the outer membrane, maltose-binding protein (MBP) in the periplasm, and the proteins of the inner membrane complex (MalFGK2), composed of one MalF, one MalG, and two MalK subunits. Subst...

Journal: :Journal of neuroimmunology 2010
K C O'Connor C Lopez-Amaya D Gagne L Lovato N H Moore-Odom J Kennedy L Krupp S Tenembaum J Ness A Belman A Boyko O Bykova J K Mah C A Stoian E Waubant M Kremenchutzky M Ruggieri M R Bardini M Rensel J Hahn B Weinstock-Guttman E A Yeh K Farrell M S Freedman M Iivanainen V Bhan M Dilenge M A Hancock D Gano R Fattahie L Kopel A E Fournier M Moscarello B Banwell A Bar-Or

Anti-myelin basic protein (MBP) antibodies in pediatric-onset MS and controls were characterized. Serum samples were obtained from 94 children with MS and 106 controls. Paired CSF and serum were obtained from 25 children with MS at time of their initial episode of acute demyelinating syndrome (ADS). Complementary assays were applied across samples to evaluate the presence, and the physical bind...

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