The binding of hepatic lipase to rat liver was studied in an ex vivo perfusion model. The livers were perfused with media containing partially purified rat hepatic lipase or bovine milk lipoprotein lipase. The activity of the enzymes was determined in the perfusion media before and after passage through the liver. During perfusion with a hepatic-lipase-containing medium the lipase activity in t...

Injection of steroid-free bovine follicular fluid (bFF; 2 \m=x\5 ml s.c. 12h apart) into anoestrous ewes lowered plasma FSH concentrations by 70% and after 24 h had significantly (P < 0\m=.\01)reduced the number of non-atretic follicles (\m=ge\1 mm diam.) without influencing the total number of follicles (\m=ge\1 mm diam.) compared to untreated controls. Hourly injections of FSH (10 \g=m\gi.v. ...

mrs method is one of the new geophysical methods that directly detects the groundwater. by using this method, one can determines some parameters of aquifer (porosity, permeability and transmissivity). the purpose of this research is the application of mrs method in the conductive ground. combination of mrs and rs method is used for better detection of the aquifer in the region of abyek located ...

Somatostatin-containing cells have been shown to be in close anatomic proximity to gastrin-producing cells in rat antral mucosa. The present studies were directed to examine the effect of secretin on carbachol-stimulated gastrin release and to assess the potential role of somatostatin in mediating this effect. Rat antral mucosa was cultured at 37 degrees C in Krebs-Henseleit buffer, pH 7.4, gas...

The purpose of this study was to develop a protocol for the micropropagation of Pinus taeda from juvenile material. Apical shoots and nodal segments were inoculated into MS, DCR or WV medium. After 90 days, the explants were transferred to WV5 medium supplemented or not with 6-benzyladenine (BA) (2.0 μM) in order to induce multiple shoot formation. For root induction, a medium composed of water...

DNA is generally considered to be chemically rather inert, but self-cleaving DNA molecules have recently been isolated from in vitro selection experiments; their properties advance the idea that, like RNA, DNA can serve as both a catalyst and an information storage medium.

The effects of concentrations of glycerol, ethylene glycol or dimethylsulphoxide (DMSO) in the presence of either 0.25 M lactose or sucrose on the post-thaw survival of mouse quickly-frozen compacted morulae were studied. In this method, the embryos were directly frozen in liquid nitrogen (LN2) vapor at approximately -170 degrees C for 2 min before being plunged into LN2. High survival rates of...

*Corresponding author: Mailing address: Kanagawa Prefectural Institute of Public Health, Shimomachiya 1-3-1, Chigasaki, Kanagawa 253-0087, Japan. Tel: +81-467-83-4400, Fax: +81467-83-4457, E-mail: [email protected] We have been closely following the trend of Mycoplasma pneumoniae infections in Kanagawa Prefecture since 1976. We report here its isolation rates and the frequency of ma...

This study evaluated the necessity and the contribution of solid media when used in conjunction with radiometric Middlebrook 7H12 (BACTEC 12B; Becton Dickinson, Towson, Md.) medium for recovery and complete identification of mycobacteria. Each of 1,184 digested, decontaminated respiratory specimens was inoculated into one BACTEC 12B vial, one 7H11 plate, and two Lowenstein-Jensen (LJ) slants. W...

We have compared the ability of the Mycobacterium Growth Indicator Tube (MGIT) system, a new culture method with an oxygen-sensitive fluorescent sensor, to recover mycobacteria from sputum samples with the abilities of egg-based medium and the Septi-Chek AFB system. We have also assessed the clinical utility of the AccuProbe or the AMPLICOR-PCR assay to directly identify Mycobacterium tuberculo...