DNA replication is a fundamental biological process. The initial step in eukaryotic DNA replication is the assembly of the pre-initiation complex, including the formation of two head-to-head hexameric helicases around the replication origin. How these hexameric helicases interact with their origin dsDNA remains unknown. Here, we report the co-crystal structure of the SV40 Large-T Antigen (LT) h...

Fundamentals of femtosecond laser pulse and nanoparticles are analyzed by a two-temperature model. Ultrafast surface melting, surface nanoengineering and shock wave impact are evident in the surface of graphite by femtosecond irradiation. The interaction between femtosecond laser pulses and Au/Ag nanoparticles has been investigated. Two effects are identified at different intensities: photofrag...

The recent increase in number and capacity of special melting techniques and refi ning processes is related to the continuing growth of high-temperature alloy usage in wrought and cast form and to the fact that the metallurgical and technological potential of conventional melting and casting processes have been largely exhausted. The evol ution of the major melting processes for high-performanc...

Abstract Changes in the Antarctic ice sheet play a critical role Southern Ocean and global climates. Although many studies have pointed out that enhanced ocean heat delivery onto continental shelf regions can cause significant changes ice-shelf basal melting, associated physical mechanisms require further research. Here, we perform numerical experiments using an ocean–sea model with component t...

The accurate prediction and characterization of DNA melting domains by computational tools could facilitate a broad range of biological applications. However, no algorithm for melting domain prediction has been available until now. The main challenges include the difficulty of mathematically mapping a qualitative description of DNA melting domains to quantitative statistical mechanics models, a...

Legionella pneumophila RpoS (LpRpoS), an alternative sigma factor of RNA polymerase (RNAP), is essential for virulence and stress resistance. To investigate the mechanism of RpoS in the intracellular pathogen L. pneumophila, we determined the high-resolution crystal structure of the LpRpoS 95–195 containing a partial region 1.2 and region 2. The structure of LpRpoS 95–195 reveals that the conse...

Legionella pneumophila RpoS (LpRpoS) is an alternative sigma factor of RNA polymerase 13 (RNAP) essential for virulence and stress resistance. To investigate the mechanism of RpoS in the 14 intracellular pathogen L. pneumophila, we determined the high-resolution crystal structure of the 15 LpRpoS (residues 95-194) containing a partial region 1.2 and region 2. The structure of LpRpoS 16 (residue...

This study addresses the crystallization and multiple melting behavior of a new high temperature semicrystalline polyimide, which is based on an ether diamine (TPER or 1,3 (4) APB) and BTDA dianhydride, both of which are commercially available. It also uses phthalic anhydride to endcap the chains thus improving thermal stability. The polyimide displays a Tg at ca. 230°C and two prominent meltin...

A hydrodynamic model based on transport equations and semi-empirical multiphase equations-of-state for metals is developed and applied to simulation of short pulse laser melting and resolidification. New computational algorithms are developed for modeling of two-phase zones of solid-liquid and liquid-gas coexistence, as well as for explicit tracking of interfaces between the phases. The model a...