The aims of the present study were to provide accurate prevalence of acute and occult hepatis B infection and hepatis C infection among potential blood donors in Cambodia and to study the accuracy of ELISA tests used for blood donor screening. A cross-sectional study was performed on samples collected from potential volunteer blood donors (n = 1,200) in two districts in rural Cambodia. The samp...

conclusions we introduced an anti-hbsp antibodies elisa, designed for locally circulating hbv strains. correlation observed of anti-hbsp with liver fibrosis staging regardless of viral replication and liver inflammation suggests anti-hbsp antibodies as possible indicator for hbv-associated liver fibrosis. results seven out of eighty (9%) chb patients were positive for anti-hbsp antibodies. mean...

background: anti-ccp is a test commonly used for the diagnosis of rheumatoid arthritis. the aim of this study was to determine the diagnostic values of accp compared to anti-mcv in rheumatoid arthritis patients in north of iran. methods: the serum samples of 150 ra patients and 75 controls, with the mean age of 49.6+11.8 and 48.8+12 years respectively, were tested using the commercially availab...

This paper describes a protocol for the preparation of highly purified A (A1 and A2) and B chains of the plant toxin, ricin, and biochemical and biological characterization of these proteins. Intact ricin was bound to acid-treated Sepharose 4B and was split on the column into A and B chains with 2-mercaptoethanol. The A chains were eluted with borate buffer containing 2-mercaptoethanol. A1 and ...

AIM The present study was carried out to evaluate the effect of supplementation of β-mercaptoethanol (β-ME) on in vitro maturation rate and glutathione (GSH) level of buffalo oocytes. MATERIALS AND METHODS Oocytes were recovered from buffalo's ovaries collected from government approved slaughter house (near Kamela darwaza, Surat) of Surat Municipal Corporation. The obtained oocytes were in vi...

The aim of this study was to determine whether more than once freezing and thawing of serum affects different patterns of anti-nuclear antibodies (ANA) and anti-neutrophil cytoplasmic antibodies (ANCA) as also to determine for how many days can serum samples be reliably refrigerated (2-8oC) for these tests. A total of 20 ANA (6 homogenous, 3 speckled, 3 centromere, 2 nucleolar, 2 cytoplasmic an...

Hepatitis E virus (HEV) is largely responsible for water borne epidemics in many developing countries. The principle mode of HEV transmission is the fecal oral route in epidemic and sporadic forms with a high case fatality ratio in pregnant women. Serum samples from 50 healthy subjects and from 435 acute viral hepatitis patients, 4-75 years old, were screened for markers of acute viral hepatiti...