Double-stranded RNA (dsRNA) inhibits expression of homologous genes by a process involving messenger RNA degradation. To gain insight into the mechanism of degradation, we examined how RNA interference is affected by mutations in the smg genes, which are required for nonsense-mediated decay. For three of six smg genes tested, mutations resulted in animals that were initially silenced by dsRNA b...

Background: RNA interference (RNAi) is the mechanism of gene silencing-mediated messenger RNA degradation by small interference RNA (siRNA), which becomes a powerful tool for in vivo research, especially in the areas of cancer. In this research, the potential use of an expression vector as a specific siRNA producing tool for silencing of Bcr-abl in K562 cell line has been investigated. Methods:...

Two distinct forms of globin messenger RNA were isolated from mouse spleen cells infected with Friend erythroleukemia virus: polyribosomal messenger ribonucleoprotein particles (15S mRNP), and their corresponding protein-free mRNAs obtained by chemical deproteinization. The translation efficiencies of both messenger forms were assayed in a Krebs II ascites cell-free system. Selective removal of...

The synthesis of deoxynucleotide kinase (EC 2.7.4.2) in vitro by a preparation consisting of T4 bacteriophage DNA and a cell-free extract of Escherichia coli has been reported. A study of the role of monovalent cations in the synthesis of this enzyme as well as alpha-glucosyl transferase (EC 2.4.1.2) shows that potassium ions are required for maximal enzyme production. Examination of the RNA-di...

BACKGROUND Scar formation and subglottic stenosis often cause health problems in surgical otolaryngology. However, fetal wounds demonstrate scarless healing. The underlying mechanism remains poorly understood. We isolated differentially expressed genes by comparison between nonwounded with wounded skin of fetal and adult rabbits. METHODS Skin incisional wounds were made in fetal (21 to 23 day...

Messenger RNA was prepared from developing sea urchin gastrulae by puromycin release from polyribosomes. Approximately 60% of the total mRNA radioactivity of the postnuclear supernatant was recovered and shown to be free of any other labeled RNA species such as ribosomal and nuclear RNA. The mRNA was examined by hybridization to DNA present in great excess. The mRNA hybridizes almost exclusivel...

Treatment of exponentially growing Novikoff rat hepatoma cells with cytostatic concentrations of phenethyl alcohol causes a rapid disappearance of both the free and the membrane-bound polyribosomes, with the concomitant appearance of 80 S and 110 S ribosomal particles. This is accompanied by an 80 to 90% reduction in the rate of protein synthesis by the cells. The phenethyl alcohol-induced loss...