Growth of the yeast Pichia pastoris on methanol induces the expression of genes whose products are required for its metabolism. Three of the methanol pathway enzymes are located in an organelle called the peroxisome. As a result, both methanol pathway enzymes and proteins involved in peroxisome biogenesis (PEX proteins) are induced in response to this substrate. The most highly regulated of the...

Formaldehyde is a prevalent environmental toxin and a key intermediate in single carbon metabolism. The ability to monitor formaldehyde concentration is, therefore, of interest for both environmental monitoring and for metabolic engineering of native and synthetic methylotrophs, but current methods suffer from low sensitivity, complex workflows, or require expensive analytical equipment. Here w...

Alterations in the steady state level of rat brain biogenic amines - dopamine, nor-epinephrine, epinephrine, serotonin and 5-hydroxy indole acetic acid, in response to intraperitoneal administration of methanol (3g/kg b.w.) were studied in discrete areas of the rat brain. The monoamine changes induced by methanol were quite different from those induced by ethanol consumption. They were also reg...

methanol is responsible for a life-threatening poisoning. fomepizole, a potent alcohol dehydrogenase inhibitor, is an efficient and safe antidote that prevents or reduces toxic methanol metabolism. although no study has compared its efficacy with ethanol, fomepizole is recommended as a first-line antidote. treatment should be started as soon as possible, based on history and initial findings in...

background: in iran, methyl alcohol poisoning rarely occurs accidentally and usually occurs through abuse of contaminated ethanol. however, with proper and timely treatment complications and mortality of methanol poisoning can be prevented. in this study, the epidemiological profile of methanol poisoning in the iranian society was investigated. methods: in this prospective cross-sectional study...

background consumption of home-distilled alcohol may lead to epidemic or sporadic cases of severe acute methanol poisoning. the difficulty of establishing strict indications for hemodialysis in acute methanol poisoning is a widely recognized issue. materials and methods the determination of the clinical, especially hemodialysis, and para clinical factors influencing patient survival in 46 acute...

Electron solvation in methanol anion clusters, (MeOH)(n) (-) (n approximately 70-460), is studied by photoelectron imaging. Two isomers are observed: methanol I, with vertical binding energies (VBE) ranging from 2-2.5 eV, and methanol II, with much lower VBE's between 0.2 and 0.5 eV. The VBE's of the two isomers depend linearly on n(-1/3) with nearly identical slopes. We propose that the excess...

Methanotrophs in enrichment cultures grew and sustained atmospheric methane oxidation when supplied with methanol. If they were not supplied with methanol or formate, their atmospheric methane oxidation came to a halt, but it was restored within hours in response to methanol or formate. Indigenous forest soil methanotrophs were also dependent on a supply of methanol upon reduced methane access ...

Effects of methanol on colony-formation of human hepatoma cells were investigated. Among five human hepatoma cell lines (Hep G2, HLE, HuH-6, HuH-7, and PLC/PRF/5), only HLE cells showed enhanced colony formation due to methanol. The effective concentrations of methanol were around 1%. The enhancement occurred in a greater degree when the cells were seeded in the culture medium containing methan...

J ^ ^^ r r I c Accepted for publication July 39, 1971 C3 A C MAJCKIDwIQ, Edwina Arm JAcx H. Mai+mzzsoN : Blood methanol corcentrntlone during experimentally induced ethanol intoxication in alcoholics. J. Pbarmacol. Exp. Ther. 179: 29;300, 1971. Accumulation of methanol in blood was detected in alcoholic subjects during a 10to 15-day period of chronic alcohol intake. Blood methanol levels increa...