In order to design a biocatalyst for the production of optically pure styrene oxide, an important building block in organic synthesis, the metabolic pathway and molecular biology of styrene degradation in Pseudomonas sp. strain VLB120 was investigated. A 5.7-kb XhoI fragment, which contained on the same strand of DNA six genes involved in styrene degradation, was isolated from a gene library of...

Atom transfer radical polymerization (ATRP) was applied to homo and block copolymerization of vinyl monomers methacrylates, acrylates, and styrene with iron (FeCl2.4H2O) as the transition metal in most cases. As complexing ligand either a commercially available ligand (triphenyl phosphine) (PPh3) or synthetic aliphatic amines were used. As initiators, methyl 2-bromopropionate, ethyl 2-bromoisob...

Styrene is a high production chemical; the production capacity for styrene in the United States was over 12 billion pounds in 2008. Small amounts of styrene are naturally present in foods such as legumes, beef, clams, eggs, nectarines, and spices. It can also be present in packaged foods by migration from polystyrene food containers and packaging materials. Styrene is a combustion product of ci...

Styrene is efficiently metabolized to styrene oxide, which is itself readily detoxified by the same enzymes as those involved in the metabolism of various foodstuffs. Styrene oxide, like many intermediate metabolites of foodstuffs, is genotoxic and, if introduced directly into the stomachs of rodents in high doses/concentrations, gives rise to cancers of the forestomach. Exposing mice to doses ...

objectives: this study aimed to compare the shear bond strength (sbs) of composite to stainless steel crowns (ssc) using two mechanical surface treatments (msts) and two bonding systems. materials and methods: eighty-four sscs were divided into six groups of 14; group1: no mst+scotchbond universal adhesive (n+u), group 2: surface roughening by a diamond bur+scotchbond universal adhesive (r+u), ...

Styrene is pneumotoxic in mice. It is metabolized by pulmonary microsomes of both mouse and rat to styrene oxide (SO), presumed to be the toxic metabolite of styrene, and known to be genotoxic. To determine which pulmonary cell types are responsible for styrene metabolism, and which cytochromes P450 are associated with the bioactivation of styrene, we isolated enriched fractions of mouse and ra...

BACKGROUND The purpose of this study was to provide some evidence of the validity of a modified shuttle test (MST) by comparing performance on the MST with peak oxygen consumption (VO2peak) measured during a treadmill test in a group of adult patients with cystic fibrosis. METHOD Twenty patients with stable cystic fibrosis performed a ramped maximal treadmill test (STEEP protocol) and the MST...

Background—The purpose of this study was to provide some evidence of the validity of a modified shuttle test (MST) by comparing performance on the MST with peak oxygen consumption (V~O2peak) measured during a treadmill test in a group of adult patients with cystic fibrosis. Method—Twenty patients with stable cystic fibrosis performed a ramped maximal treadmill test (STEEP protocol) and the MST ...

BACKGROUND AND OBJECTIVE To reduce imaging time and complexity, we sought to determine whether single breath-hold, multi-slice TrueFISP (SB-MST) magnetic resonance imaging (MRI) method is comparable to standard multi-breath-hold, multi-slice TrueFISP (MB-MST) for assessment of left ventricular (LV) wall motion abnormality (WMA), volumes, and ejection fraction (EF). METHODS AND RESULTS We stud...