The aim of this study was to evaluate the effects of the laser radiation (685 nm) associated with photosensitizers on viability of different species of Candida genus. Suspensions of Candida albicans, Candida dubliniensis, Candida krusei and Candida tropicalis, containing 10(6) viable cells per milliliter were obtained with the aid of a Neubauer's chamber. From each species, 10 samples of the ce...

Procedures with potential value for identification and purification of Mycoplasma were applied to over 240 cultures representing 11 of the 12 known avian serotypes. Growth was tested at pH 5.5 and 9.5, at 25 and 42 C, in serum-free media, and in the presence of (i) 1% bile salts, (ii) 3% NaCl, and (iii) 0.02% methylene blue. One avian Mycoplasma serotype grew in broth containing 1% bile salts. ...

Neural stem cell-based treatment holds a new therapeutic opportunity for neurodegenerative disorders. Here, we investigated the effect of methylene blue on proliferation and differentiation of rat neural progenitor cells (NPCs) both in vitro and in vivo. We found that methylene blue inhibited proliferation and promoted quiescence of NPCs in vitro without affecting committed neuronal differentia...

Methylene blue-treated fresh-frozen plasma (MB-FFP) is mainly used in Europe. The advantage of the methylene blue system is that units can be treated individually. The combined action of methylene blue and illumination is a photodynamic process preventing viral RNA and DNA replication. We report the first immediate allergic hypersensitivity reaction to methylene blue-treated plasma transfusion....

THREE types of reaction which will bring about the reduction of methylene blue added to milk have been known for a considerable time and have been utilised in various ways. (i) Schardinger [1902] has shown that milk contains an enzyme which is able to catalyse the reduction of methylene blue in the presence of an aldehyde. Trommsdorff [1909] has proved that the enzyme is a constituent of the mi...

The objective of the present study was to establish whether high-density lipoprotein 3 (HDL3) or high-density lipoprotein 2 (HDL2) might show an anti-oxidative effect on the acceleration of the oxidative modification of low-density lipoprotein (LDL) by ascorbic acid from measurement of the agarose gel electrophoretic mobility of LDL. LDL was incubated without adding transitional-metal ions for ...

In 1928 Miiller (1) reported the presence of a new enzyme, a glucose oxidase, in press-juice of Aspergillus niger. He subsequently suggested that it might be a dehydrogenase (2) ; he later showed that it catalyzed the oxidation of glucose to gluconic acid (3). Harrison in 1931 obtained glucose dehydrogenase from acetone-dried mammalian liver by saturating a water extract with ammonium sulfate (...

Angiogenesis is the process of generating new blood vessels from preexisting vessels and is considered essential in many pathological conditions. The purpose of the present study was to evaluate the effect of methylene blue in chick chorioallantoic membrane angiogenesis model in vivo. In this well characterized model, methylene blue inhibited angiogenesis in a concentration-dependent manner. In...

The use of biodegradation methods by microorganisms in the removal of industrial dyes are widely considered owing to their high efficiency and compatibility to the environment. Therefore, this study aims to evaluate the biodegradation of methylene blue (MB) from aqueous solution by bacteria isolated from contaminated soil. This study was performed in laboratory scale on bacteria isolated and pu...

Methylene blue is an intravenously administered agent that may potentiate serotonin syndrome. The usage of methylene blue to evaluate ureters for injuries and patency during urological surgeries is recognized as common practice. However, there is no mention of serotonin syndrome caused by methylene blue in urological literature or for urological surgery. We report the first urological case in o...