We report here the development of 65 novel microsatellite loci and construction of a composite genetic linkage map for Culex pipiens complex mosquitoes. Microsatellites were identified by in silico screening of the Culex quinquefasciatus genome assembly. Cross-species utility of 73 microsatellites for population studies in C. pipiens sensu stricto and C. quinquefasciatus was evaluated by genoty...

We investigate the interplay between genetic diversity and recombination in maize (Zea mays ssp. mays). Genetic diversity was measured in three types of markers: single-nucleotide polymorphisms, indels, and microsatellites. All three were examined in a sample of previously published DNA sequences from 21 loci on maize chromosome 1. Small indels (1-5 bp) were numerous and far more common than la...

An important emerging application of high-throughput 454 sequencing is the isolation of molecular markers such as microsatellites from genomic DNA. However, few studies have developed microsatellites from cDNA despite the added potential for targeting candidate genes. Moreover, to develop microsatellites usually requires the evaluation of numerous primer pairs for polymorphism in the focal spec...

It is well known that human embryonic stem cells (hESCs) frequently acquire recurrent chromosomal abnormalities, very reminiscent of those found in cancerous cells. Given the parallels between cancer and stem cell biology, we set out to investigate the occurrence of a common form of genome instability in tumors, namely microsatellite instability (MSI), in hESCs. MSI is caused by a deficiency in...

The ETS gene family is frequently involved in chromosome translocations that cause human cancer, including prostate cancer, leukemia, and sarcoma. However, the mechanisms by which oncogenic ETS proteins, which are DNA-binding transcription factors, target genes necessary for tumorigenesis is not well understood. Ewing's sarcoma serves as a paradigm for the entire class of ETS-associated tumors ...

Recent improvements in next-generation sequencing technologies have enabled investigation of microsatellites on a genome-wide scale. Faced with a huge amount of candidates, the use of appropriate marker selection criteria is crucial. Here, we used the western flower thrips Frankliniella occidentalis for an empirical microsatellite survey and validation; 132,251 candidate microsatellites were id...

for identification of individuals and parentage control performed by cattle breeders in the czech republic, a novel finnish bovine genotypes™ panel 3.1was amplified by means of one multiplex polymerase chain reaction. bovine panel encompasses all the 12 str loci recommended by the international society for animal genetics (isag) for routine use in parentage testing and identification, including...

Microsatellites are the kinds of sequences in the genome of living organism that have the fastest variation frequency. The variations of numbers of microsatellite repeat units in the structure gene cause frame-shift mutation of the gene, resulting in gene expressing in fully difference or expressing truncated protein. Thus, in the evolutionary process, microsatellite within the gene region woul...

Abstract With the increasing development of aerospace technology, micro satellites have attracted high attention in field due to their advantages such as short cycle, low system investment, and flexible launch methods. Their level internal equipment integration, heat flux density, complex uncertain working modes pose new challenges thermal control design. At present, design still mainly adopts ...

Abstract Small and isolated populations face several intrinsic risks, such as genetic drift, inbreeding depression reduced gene flow, patterns of diversity differentiation have become an important focus conservation genetics research. The golden snub-nosed monkey Rhinopithecus roxellana, endangered species endemic to China, has experienced rapid reduction in population size severe fragmentation...