The environmental DNA (eDNA) method is a detection technique that is rapidly gaining credibility as a sensitive tool useful in the surveillance and monitoring of invasive and threatened species. Because eDNA analysis often deals with small quantities of short and degraded DNA fragments, methods that maximize eDNA recovery are required to increase detectability. In this study, we performed exper...

The stomach, which is characterized by acid peptic digestion in vertebrates, has been lost secondarily multiple times in the evolution of the teleost fishes. The Cypriniformes are largely seen as an agastric order; however, within the superfamily Cobitoidea, the closely related sister groups Nemacheilidae and Balitoridae have been identified as gastric families. The presence of these most recen...

The following caryophyllidean tapeworms were found in freshwater fishes from Japan (species reported from Japan for the first time marked with an asterisk): family Caryophyllaeidae: Paracaryophyllaeus gotoi (Motomura, 1927) from Misgurnus anguillicaudatus (Cantor); Archigetes sieboldi Leuckart, 1878 from Pseudorasbora parva (Temminck et Schlegel) and Sarcocheilichthys variegatus microoculus Mor...

RNA sequencing and short-read assembly were utilized to produce a transcriptome of livers from loaches (Misgurnus anguillicaudatus) fed with three different diets respectively containing fresh fish oil (FO group), medium oxidized fish oil (MO group) and high oxidized fish oil (HO group). A total of 60,663 unigenes were obtained in this study, with mean length 848.74 bp. 50,814, 49,584 and 49,81...

Temperature has significant influence on physio-chemical responses of all living organisms including fish. The present study was conducted to evaluate the effect of temperature on dimethoate toxicity (96 h static bioassay) to freshwater catfish Heteropneustes fossilis. Fish mortality data were recorded at 24, 48, 72 and 96 h and analysed using EPA Probit analysis, Version 1.5, software based on...

As a forward step to understand the metal bioaccumulation in fish species, a study of trace metal speciation of sediment was achieved. Trace metals were measured in sediments and two fish species, Heteropneustes fossilis and Trichogaster fasciata by using inductively coupled plasma mass spectrometer (ICP-MS). Abundance of total trace metals in sediments varied in the following decreasing order:...

The construction of a reservoir dam alters the environment within its basin, including composition fish community, biodiversity, and river ecosystem itself. This study was conducted in Sanbanxi Reservoir used eDNA metabarcoding technology comprising capture extraction, PCR amplification, sequencing database comparison analysis, other environmental DNA standardized analysis processes to characte...

Basic and neutral neurohypophyseal (NH) nonapeptides have evolved from vasotocin (VT) by a gene duplication at the base of the gnathostome lineage. In teleosts, VT and IT are the basic and neutral peptides, respectively. In the present study, VT and IT precursor genes of Heteropneustes fossilis and Clarias batrachus (Siluriformes, Ostariophysi) were cloned and sequenced. The channel catfish Ica...