Addition of n-butyl nitrite to isolated rat hepatocytes caused an immediate glutathione depletion followed by an inhibition of mitochondrial respiration, inhi- bition of glycolysis and ATP depletion. At cytotoxic butyl nitrite concentrations, lipid  peroxidation  occurred  before  the  plasma  membrane  was  disrupted. Cytochrome P-450 inhibitors inhibited peroxynitrite formation and prev...

There are currently 25 recognized species of the chipmunk genus Tamias. In this study we sequenced the complete mitochondrial cytochrome b (cyt b) gene of 23 Tamias species. We analyzed the cyt b sequence and then analyzed a combined data set of cyt b along with a previous data set of cytochrome oxidase subunit II (COII) sequence. Maximum-likelihood was used to further test the fit of models of...

Multiple Sclerosis (MS) is a neurodegenerative and autoimmune disease that it’s molecular etiology and factors involving in its progression remains unknown. In this study for evaluation effect of mercuric on progression of MS we investigated the additive effect of mercuric sulfide on the brain mitochondrial dysfunction in experimental autoimmune encephalomyelitis (EAE) model of MS in C57BL/6 mi...

background : phlebotomus sergenti s.l. is considered the most likely vector of leishmania tropica in iran. although two morphotypes- p. sergenti sergenti (a) and p. sergenti similis (b)-have been formally described, further morphologi­cal and a molecular analysis of mitochondrial cytochrome oxidase i (mtdna-coi) gene revealed inconsistencies and suggests that the variation between the morphotyp...

Mitochondrial DNA (mtDNA) is highly susceptible to mutations due to the low level of DNA repair and the presence of a high level of reactive oxygen species in the organelle. Although mtDNA mutations have been implicated in degenerating diseases, aging, and cancer, very little is known about the role of T cells in immunosurveillance for mtDNA aberrations. Here, we describe T-cell recognition of ...

Mitochondrial oxidative phosphorylation (OXPHOS) is responsible for generating the majority of cellular ATP. Complex III (ubiquinol-cytochrome c oxidoreductase) is the third of five OXPHOS complexes. Complex III assembly relies on the coordinated expression of the mitochondrial and nuclear genomes, with 10 subunits encoded by nuclear DNA and one by mitochondrial DNA (mtDNA). Complex III deficie...

purpose : to investigate the effects of mtp-131 on trabeculum cells cultured under hydrostatic pressure methods : glaucomatous human trabecular meshwork (gtm3) cells were divided into three groups: the mtp-131 group, the hydrostatic group, and the normal group. the mtp-131 group was pretreated with 1 µm mtp-131, and the cells were then cultured under 80 mmhg pressure for 24 hours. the normal gr...

background: molecular diversity of leishmania major and its morphological changes have become a controversial issue among researchers. some aspects of polymorphic shapes of amastigotes in clinical manifestations along with molecular variation were evaluated among suspected patients of some exceptional zoonotic cutaneous leishmaniasis locations in northern khuzestan, southwestern iran. methods: ...

multiple sclerosis (ms) is a neurodegenerative and autoimmune disease that it’s molecular etiology and factors involving in its progression remains unknown. in this study for evaluation effect of mercuric on progression of ms we investigated the additive effect of mercuric sulfide on the brain mitochondrial dysfunction in experimental autoimmune encephalomyelitis (eae) model of ms in c57bl/6 mi...

cigarette smoking is one of the main risk factors for premature human death associated to a variety of respiratory and vascular diseases, and cancer due to containing hundreds of toxicants. rat mitochondria were obtained by differential ultracentrifugation and incubated with different concentrations (1, 10 and 100%) of standardized cigarette smoke extract (cse). our results showed that cigarett...