Epithelioid sarcoma (ES) is rare with a poor prognosis and for which a loss of INI1 expression has been recently reported. We report a study of 106 cases with clinical, histologic, and immunohistochemical data, including INI1 expression, and follow-up data. Of the 106 cases, 70 were the conventional subtype and 36 the large cell subtype. INI1 was negative in 86 cases (81.1%): 57 (81%) of 70 con...

Male Wistar rats weighing 100 g received 1,2-dimethylhydrazine (25 mg/kg) s.c.) twice a week for 2 months and once a week thereafter for an additional 4 months. Groups of four to six rats were sacrificed monthly. Paraffin sections of duodenum were prepared and stained with periodic acid-Schiff and hematoxylin for cell counts and with toluidine blue for measuring nucleolar area. As an index of v...

AIMS To determine the prognostic usefulness of the Nottingham histological grade (NHG) and its components in a series of 270 patients with stage pT1N0M0 breast cancer with a median follow up of 12.5 years. METHODS Microscopic slides were re-examined and the degree of tubule formation, nuclear pleomorphism, and mitotic counts were assessed and scored according to the suggested guidelines. The ...

INTRODUCTION Mitotic Activity Index (MAI) is an important independent prognostic factor and an integral part of the breast cancer grading system. Thus, correct estimation of this prognostically relevant feature is essential for guiding treatment decision and assessing patient prognosis. The aim of this study was to validate the use of high resolution Whole Slide Images (WSI) in estimating MAI i...

Eukaryotic cells have evolved a mechanism that delays the progression of mitosis until condensed chromosomes are properly positioned on the mitotic spindle. To understand the molecular basis of such monitoring mechanism in human cells, we have been studying genes that regulate the mitotic checkpoint. Our early studies have led to the cloning of a full-length cDNA encoding MAD3-like protein (als...

Calotropis procera is a tropical medicinal plant known for its multiple curative effects on wide range of diseases. Current study has evaluated the antimitotic activity of the extracts of leaf and latex of C. procera on root meristem cells of Allium cepa. Onion bulbs were allowed to grow roots on moist cotton imbibed with solutions of test sample with known concentration of the extracts. Mitoti...

Topical diclofenac in 2.5% hyaluronan inhibits basal cell carcinoma, actinic keratosis, and murine colon-26 growth in vivo. colon-26 tumor growth was preceded by angiogenesis and reduced apoptotic and mitotic indices. Diclofenac reduced proliferation and viability in vitro, and stimulated apoptosis. Hyaluronan inhibited proliferation and viability at 1 mg/ml but was inactive below this level. T...

Any deviation in ambient temperature causes stress and disturbs the cellular homeostasis in the organisms. In the present study, genotoxicity induced by exposure of high temperature (40o, 45o and 50oC) has been analyzed in the third instar larvae of common housefly, Musca domestica, by using chromosome aberration assay, micronucleus assay and mitotic index as cytological end points. The adverse...

In this study, the mutagenic effects of low power radiofrequency radiation on Zea mays root tip were studied. Cells in different division phases and chromosomal aberration assay were used to determine the mitotic index and chromosomal aberration frequency of Zea mays root tip cells induced by 900MHz radiofrequency radiation. Zea mays seeds, having a uniform genophond, have been exposed to RF fi...

Recently, mitotic arrest deficient 2 (MAD2)-mediated spindle checkpoint is shown to induce mitotic arrest in response to DNA damage, indicating overlapping roles of the spindle checkpoint and DNA damage checkpoint. In this study, we investigated if MAD2 played a part in cellular sensitivity to DNA-damaging agents, especially cisplatin, and whether it was regulated through mitotic checkpoint. Us...