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introduction: extracellular matrix (ecm) components significantly influence the growth characteristics of cell, development of spontaneous contractile activity, andmorphologic differentiation. the present study evaluates the effect of ecm ongenotypic and physiologic characteristics of cardiomyocytes derived from embryonic stem (es) cells. material and methods: embryoid bodies derived from mouse...

Although the fibroblast growth factor (FGF) signaling axis plays important roles in cell survival, proliferation, and differentiation, the molecular mechanism underlying how the FGF elicits these diverse regulatory signals is not well understood. By using the Frs2α null mouse embryonic fibroblast (MEF) in conjunction with inhibitors to multiple signaling pathways, here we report that the FGF si...

Listeria monocytogenes insertion mutants defective in hemolysin production were generated using the conjugative transposons Tn916 and Tn1545. All of the nonhemolytic mutants (hly-) lacked a secreted 58-kD polypeptide, presumedly hemolysin, and were avirulent in a mouse model. An intracellular multiplication assay was established in monolayers of mouse bone marrow-derived macrophages, the J774 m...

Chinese hamster lung fibroblast V79 cells have been widely used in studies of DNA damage and DNA repair. Since the p53 gene is involved in normal responses to DNA damage, we have analyzed the molecular genetics and functional status of p53 in V79 cells and primary Chinese hamster embryonic fibroblast (CHEF) cells. The coding product of the p53 gene in CHEF cells was 76 and 75% homologous to hum...

G-protein-coupled receptors (GPCRs) mostly signal through heterotrimeric G proteins. Increasing evidence suggests that GPCRs could function in a G-protein-independent manner. Here, we show that at low concentrations of an agonist, beta(2)-adrenergic receptors (beta(2)-ARs) signal through Galpha(s) to activate the mitogen-activated protein kinase pathway in mouse embryonic fibroblast cells. At h...

The Rockefeller University Press $30.00 J. Cell Biol. Vol. 207 No. 5 627–642 www.jcb.org/cgi/doi/10.1083/jcb.201405105 JCB 627 *B.K. Tripathi and X. Qian contributed equally to this paper. Correspondence to Douglas R. Lowy: [email protected]; or Brajendra K. Tripathi: [email protected] Abbreviations used in this paper: HBEC, human bronchial epithelial cell; IB, immunoblotting; IP, immunop...

The preparation of highly stable water dispersions of silver nanoparticles using the naturally available gellan gum as a reducing and capping agent is reported. Further, exploiting the gel formation characteristic of gellan gum silver nanoparticle incorporated gels have also been prepared. The optical properties, morphology, zeta potential and long-term stability of the synthesized silver nanop...

249 words Introduction: 735 words Discussion: 1493 words d) ABBREVIATIONS: ABC, ATP-binding cassette transporter; ADO, adenosine; CFTR, cystic fibrosis transmembrane conductance regulator; EPAC, GTP-exchange protein activated by cAMP; FSK, forskolin; IBMX, 3-Isobutyl-1-methylxanthine; MEF, mouse embryonic fibroblast; MRP, multidrug resistant protein; PDE, cyclic nucleotide phosphodiesterase; PG...

Cover illustration: The cover image is based on the Research Article Repetitive short-pulsed illumination efficiently activates photoactivatable-Cre as continuous in embryonic stem cells and pre-implantation embryos of transgenic mouse by Kanae Kishi et al., https://doi.org/10.1002/dvg.23457.