نتایج جستجو برای: mouse somatic cell nuclear transfer

تعداد نتایج: 2294906  

Journal: :Biology of reproduction 2002
Shoukhrat M Mitalipov Richard R Yeoman Kevin D Nusser Don P Wolf

Production of genetically identical nonhuman primates would reduce the number of animals required for biomedical research and dramatically impact studies pertaining to immune system function, such as development of the human-immunodeficiency-virus vaccine. Our long-term goal is to develop robust somatic cell cloning and/or twinning protocols in the rhesus macaque. The objective of this study wa...

2016
Lin Bai Mengqi Li Junli Sun Xiaogan Yang Yangqing Lu Shengsheng Lu Kehuan Lu

The specific molecular mechanisms involved in somatic reprogramming remain unidentified. Removal of the oocyte genome is one of the primary causes of developmental failure in cloned embryos, whereas intact oocyte shows stronger reprogramming capability than enucleated oocyte. To identify the reason for the low efficiency of cloning and elucidate the mechanisms involved in somatic reprogramming ...

Journal: :Theriogenology 2008
H J Oh M K Kim G Jang H J Kim S G Hong J E Park K Park C Park S H Sohn D Y Kim N S Shin B C Lee

The objective of the present study was to investigate whether nuclear transfer of postmortem wolf somatic cells into enucleated dog oocytes, is a feasible method to produce a cloned wolf. In vivo-matured oocytes (from domestic dogs) were enucleated and fused with somatic cells derived from culture of tissue obtained from a male gray wolf 6h after death. The reconstructed embryos were activated ...

Journal: :Frontiers in ophthalmology 2023

Starburst amacrine cells are a prominent neuron type in the mammalian retina that has been well-studied for its role direction-selective information processing. One specific property of these is their dendrites tightly stratify at depths within inner plexiform layer (IPL), which, together with unique expression choline acetyltransferase (ChAT), made them most common depth marker studying other ...

2014
Gareth D. Greggains Lisa M. Lister Helen A. L. Tuppen Qi Zhang Louise H. Needham Nilendran Prathalingam Louise A. Hyslop Lyndsey Craven Zbigniew Polanski Alison P. Murdoch Douglass M. Turnbull Mary Herbert

Induced pluripotent stem cells (iPSCs) hold much promise in the quest for personalised cell therapies. However, the persistence of founder cell mitochondrial DNA (mtDNA) mutations limits the potential of iPSCs in the development of treatments for mtDNA disease. This problem may be overcome by using oocytes containing healthy mtDNA, to induce somatic cell nuclear reprogramming. However, the exte...

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