In this study, we examined the in vitro and in vivo effects of forced expression of Mpl-R (the thrombopoietin receptor) on the progeny of murine hematopoietic stem cells. Bone marrow cells from 5-FU-treated mice were transduced with retroviral vectors containing the human Mpl-R cDNA, or the neomycine gene as a control. After 7 days cocultivation on virus-producer cells, GpE86-Mpl-R or Gp86-Neo,...

which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Is RARS-T a new disease entity or a subtype of RARS or ET? TO THE EDITOR: A recent report of " JAK2 V617F mutation in myelodysplastic syndrome, myelodysplastic syndrome/myelo-proliferative neoplasm, unclassifiable, refractory anemia with ring sideroblasts wi...

Recent studies have shown decreased megakaryocyte expression of the thrombopoietin receptor (c-mpl) in patients with polycythemia vera (PV) but not in those with reactive erythrocytosis. We examined the diagnostic utility of this observation in 22 patients with PV, 7 patients with secondary erythrocytosis (SE), and 10 normal controls. Commercial antibodies against c-mpl were used with standard ...

PURPOSE To review the most important metabolic effects and clinical safety data of subthreshold micropulse diode laser (D-MPL) in diabetic macular edema (DME). METHODS Review of the literature about the mechanisms of action and role of D-MPL in DME. RESULTS The MPL treatment does not damage the retina and is selectively absorbed by the retinal pigment epithelium (RPE). MPL stimulates secret...

Cisplatin is an effective chemotherapeutics against a wide range of cancers. However, it causes significant nausea and vomiting which limit its usefulness. In the present study, the effects of methanolic root extract of Pluchea lanceolata (DC.) C. B. Clarke , asteraceae ( MPL ) was investigated against cisplatin-induced nausea using a rat pica model. In rat pica model, rats react to cispla...

The "zinc-finger" transcription factor GATA-1 was first shown in cells of erythroid lineage. It is also expressed in cells of other hematopoietic lineages including megakaryocytes, mast cells, and eosinophils. GATA-1 is now considered to be one of the central regulators in hematopoietic cell differentiation. To further analyze the role of GATA-1 in controlling differentiation from hematopoietic...

Thrombopoietin (TPO) is a hematopoietic growth factor that plays fundamental roles is both megakaryopoiesis and thrombopoiesis through binding to its receptor, c-mpl. Although TPO has been shown to activate various types of intracellular signaling molecules, such as the Janus family of protein tyrosine kinases, signal transducers and activators of transcription (STATs), and ras, the precise mec...

BACKGROUND Somatic calreticulin (CALR), Janus kinase 2 (JAK2), and thrombopoietin receptor (MPL) mutations essentially show mutual exclusion in myeloproliferative neoplasms (MPN), suggesting that they activate common oncogenic pathways. Recent data have shown that MPL function is essential for CALR mutant-driven MPN. However, the exact role and the mechanisms of action of CALR mutants have not ...