The mutagenic potentials of ethylmethane sulfonate, .V-nu-tliyl-.V'nitrosoguanidine, and benzo(a)pyrene diol-epoxide in human mitochon dria were determined by cloning and nucleotide sequencing of mitochon dria! (mt) DNA from HeLa cells treated with these mutagens. Mutagen concentrations that reduced cell survival to approximately 0.1% of un treated cultures were used. Mitochondria! DNA was prep...

4-Nitroquinoline 1-oxide (4-NQO) is a highly carcinogenic chemical that induces mutations in bacteria, fungi, and animals through the formation of bulky purine adducts. 4-NQO has been used as a mutagen for genetic screens and in both the study of DNA damage and DNA repair. In the model eukaryote Aspergillus nidulans, 4-NQO-based genetic screens have been used to study diverse processes, includi...

Treatment of diploid human fibroblasts with an alkylating mutagen has been shown to induce stable, anchorage-independent cell populations at frequencies (11 X 10(-4) consistent with an activating mutation. After treatment of human foreskin fibroblasts with the mutagen benzo[a]pyrene (+/-)anti- 7,8-dihydrodiol 9,10-epoxide and selection in soft agar, 17 anchorage-independent clones were isolated...

Methylazoxymethanol (MAM) and dimethyl sulfate (DMS) are mutagens whose genetic effects can be ascribed to the methylation of DNA. While both methylate the N7 position of guanine heavily, only MAM strongly methylates the O(6) position of guanine. We evaluated the relative effectiveness and specificity of MAM and DMS in bacterial assays for the induction of point mutations and the formation of c...

The mutagenic potentials of ethylmethane sulfonate, N-methyl-N'-nitrosoguanidine, and benzo(a)pyrene diol-epoxide in human mitochondria were determined by cloning and nucleotide sequencing of mitochondrial (mt) DNA from HeLa cells treated with these mutagens. Mutagen concentrations that reduced cell survival to approximately 0.1% of untreated cultures were used. Mitochondrial DNA was prepared 2...

Miracil D preferentially inhibited the growth of a deoxyribonucleic acid (DNA) polymerase-deficient Escherichia coli strain. This is taken to indicate that miracil D is capable of altering the DNA of living cells and that it may thereby be a potential mutagen and carcinogen.

Specific gene systems expressed in cereal pollen could contribute uniquely to the problem of monitoring our environment for mutagens. This paper considers the development of a mutagen monitor with quantitative endpoints that reflect particular types of lesions at the DNA level, and lesions in particular components of the gene.

Çalışmada bir iyonize radyasyon kaynağı olan Cs 137 kullanılarak aspir çeşitlerinin tohumlarına 
 200,300,400,500,600 Gy dozda gama ışını uygulanmıştır. Çalışmalar Tesadüf Parselleri Deneme Desenine göre 3 tekerrürlü olarak yürütülmüştür. Çalışmalarda ışınlanmamış tohumlara da aynı zamanda uygulamalar yapılmıştır. Araştırmadan elde edilen verilerle çeşitlerin ayrı varyans analizi Aspir rej...

A key component of a sound functional genomics infrastructure is the availability of a knockout mutant for every gene in the genome. A fruitful approach to systematically knockingout genes in the plant Arabidopsis thaliana has been the use of transferred-DNA (T-DNA) from Agrobacterium tumefaciens as an insertional mutagen. One of the assumptions underlying the use of T-DNA as a mutagen is that ...

Group effort from poliovirus he pathogenesis of poliovirus relies on virus diversity per se, not the selection of any particular adaptive mutation, say Marco Vignuzzi, Raul Andino (UCSF, San Francisco, CA), and colleagues. Different variants may achieve different tasks in a group effort to navigate the host environment. This is how a quasispecies, or group of variants, is meant to behave, based...