objective(s): adeno-associated virus type 2 (aav2) vectors are widely used for both experimental and clinical gene therapy. a recent research has shown that the performance of these vectors can be greatly improved by substitution of specific surface-exposed tyrosine residues with phenylalanines. in this study, a fast and simple method is presented to generate aav2 vector helper plasmids encodin...

thirty five monoconidial isolates of the fungal culture collection pyricularia grisea were examined, to identify the vegetative compatibility groups and characterize the genetic diversity of isolates as through rep-pcr genomic fingerprinting. the isolates were collected from ricefields during 1997-1999. genetic diversity of p. grisea isolates was studied as based on dna fingerprinting through r...

in order to increase tylosin production by streptomyces fradiae, two mutagens, i. e. uv and ems (ethylmethanesulfonate) were used. death curves were plotted for these mutagens. optimum condition for obtaining mutants by uv radiation was determined by using a uv source lamp at a distance of 20 cm for 40-60 seconds. when ems was used at 4% (w/v) concentration, the best mutants were isolated after...

members of the genus bacillus are among the most promising candidates for biological control of plant pathogenic bacteria. in this study, cell free supernatants of three strains b. subtilis utb96, b. subtilis utb1 and b. subtilis utb70 against aspergillus flavus r5 were investigated using dual culture assay. strain b. subtilis utb96 showed the highest inhibition effect. thin lyer chromatography...

The absorption and utilization of NH4+ NO3− by plant roots is closely related to soil moisture. In this study, we investigated the effect short-time drought rewatering on uptake assimilation in 1-year-old Malus hupehensis plants, as well transcription changes ammonium transporters (AMTs) nitrate (NRTs). roots, content reductase activity decreased under some extent was restored after rewatering....

The present study was conducted at department of Genetics, Osmania University, Hyderabad, Telangana, India, during September to November, 2021 with the blackgram accession- IC-436524 obtained from NBPGR regional centre, India and check T9 collected ICAR-CRIDA Hyderabad. aimed estimate mutagenic sensitivity black gram Ethyl methane sulfonate. Accordingly, this checked whether activity has been i...

Vertebrate behavior is strongly influenced by light. Light receptors, encoded functional opsin proteins, are present inside the vertebrate brain and peripheral tissues. This expression feature from fishes to human appears be particularly prominent in diurnal vertebrates. Despite their conserved widespread occurrence, nonvisual functions of opsins still largely enigmatic. even more apparent when...