objective: bone marrow mesenchymal stem cells reside in bone marrow and support homing and differentiation of hematopoietic stem cells (1). this makes them an excellent instrument for regenerative treatment and co-culture with the hsc. materials and methods: in this study, k562 cell lines were treated with butyric acid(for erythroid differentiation), co-cultured with mesenchymal stem cells and ...

objective(s): carbon nanotube (cnt) has been widely applied at molecular and cellular levels due to its exceptional properties. studies based on conjugation of cnts with biological molecules indicated that biological activity is preserved. polyethylenimine (pei) is explored in designing novel gene delivery vectors due to its ability to condense plasmid dna through electrostatic attraction. in t...

Background DHA-H (Hydroxy-docosahexaenoic acid) is a molecule in development for Alzheimer’s disease (AD) treatment based on the concept of membrane lipid therapy (melitherapy). Once entered cell, metabolized via α-oxidation to fatty acid HPA (Heneicosapentaenoic acid). has been shown reduce amyloidogenic processing Amyloid Precursor Protein (APP), as well having neuroprotective effect cellular...

Co-injection of wortmannin (inhibitor of phosphatidylinositol-3 kinase, PI3K) and GF109203X (inhibitor of protein kinase C, PKC) into the rat brain was found to induce spatial memory deficiency and enhance tau hyperphosphorylation in the hippocampus of rat brain. To establish a cell model with durative Alzheimer-like tau hyperphosphorylation in this study, we treated N2a neuroblastoma cells wit...

background fish cell lines are advantageous alternatives to mammalian cell lines for carrying out in vitro research. they are used extensively for the study of fish biology, physiology and toxicology, and more recently for germplasm conservation of important fish species. objectives the present study aims to establish and characterize a novel fish cell line from muscle tissue of wallago attu ca...

While the prion protein (PrP) is clearly involved in neuropathology, its physiological roles remain elusive. Here, we demonstrate PrP functions in cell-substrate interaction in Drosophila S2, N2a and HeLa cells. PrP promotes cell spreading and/or filopodia formation when overexpressed, and lamellipodia when downregulated. Moreover, PrP normally accumulates in focal adhesions (FAs), and its down...