Feline sarcoma virus (FeSV) rescued from transformed nonproducer mink or rat cells contains two FeSV-specific antigens (p15 and p12), and the feline oncornavirus-associated cell membrane antigen (FOCMA). All three antigens are helper virus-independent and are encoded by the FeSV genome. FOCMA, pl5, and p12 antigens cochromatograph as phosphorylated molecules of 85,000 molecular weight (pp85), a...

Loss of pesticide efficacy resulting from enhanced rates of microbial degradation has been observed with several pesticides including the insecticide carbofuran (2,3-dihydro-2,2-dimethyl-7-benzofuranyl methylcarbamate). Soils in which this phenomenon occurs are often referred to as "problem soils." Several previous studies have documented the temporal aspects of the conversion of a nonproblem s...

The NN-dicyclohexylcarbodiimide (DCCD). sensitive latent adenosinetriphosphatase (ATPase)(EC 3.6.1.3; ATP phosphohydrolase) from Mycobacterium phlei has been purified to homogeneity and used to restore oxidative phosphorylation to detergent-extracted membranes. The phosphorylation was inhibited by DCCD and by tetraphenylboron and valinomycin. The enzyme was solubilized from the membrane vesicle...

Comparison of the amino acid compositions of purified proteins indicates the presence of overlapping evolutionary affinities among enzymes of the 3-ketoadipate pathway. Isofunctional enzymes from different bacterial genera share a common evolutionary origin. Moreover, enzymes that mediate isofunctional or chemically analogous reactions within an organism appear to be evolutionarily homologous. ...

(1966). 4. A. Carlsson and M. Lindqvist, Acta Pharmacol. Toxicol. 20, 40 (1963); H. Nyback and G. Sedvall, Eur. J. Pharmacol. 10, 193 (1970); N.-E. Anden, S. G. Butcher, H. Corrodi, K. Fuxe, U. Ungerstedt, ibid. 11, 303 (1970); H. H. Keller, G. Bartholini, A. Pletscher, ibid. 23, 183 (1973); A. Carlsson, in (1), p. 57. 5. C. D. Wise and L. Stein, Science 181, 344 (1973). 6. R. J. Wyatt, M. A. S...

The DNA repair protein 06-methylguanine-DNA methyltransferase (MGMT) confers resistance to therapeutic methylating and chloroethylating agents in human brain tumor-derived cell lines. In this work, we assayed MGMT activity in 152 adult gliomas to establish correlates with patient and tumor characteristics. We also assayed MGMT in histologically normal brain adjacent to 87 tumors to characterize...

Genetic studies were made on E. coli K-12 TM96, which carries recombinant molecules constructed by in vitro combination of colicin El DNA and a DNA fragment of E. coli for guanine synthesis derived from transducing phage. The recombinant molecules existed as stable plasmids within the cell and contained genes for colicin El immunity and the guaA enzyme (xanthosine 5'-monophosphate aminase) toge...

(1966). 4. A. Carlsson and M. Lindqvist, Acta Pharmacol. Toxicol. 20, 40 (1963); H. Nyback and G. Sedvall, Eur. J. Pharmacol. 10, 193 (1970); N.-E. Anden, S. G. Butcher, H. Corrodi, K. Fuxe, U. Ungerstedt, ibid. 11, 303 (1970); H. H. Keller, G. Bartholini, A. Pletscher, ibid. 23, 183 (1973); A. Carlsson, in (1), p. 57. 5. C. D. Wise and L. Stein, Science 181, 344 (1973). 6. R. J. Wyatt, M. A. S...

The DNA repair protein 06-methylguanine-DNA methyltransferase (MGMT) confers resistance to therapeutic methylating and chloroethylating agents in human brain tumor-derived cell lines. In this work, we assayed MGMT activity in 152 adult gliomas to establish correlates with patient and tumor characteristics. We also assayed MGMT in histologically normal brain adjacent to 87 tumors to characterize...

Restriction maps for 25 kilobases of DNA around the 87A7 heat shock locus have been determined in 29 chromosomes isolated from a natural population. The heterozygosity per nucleotide and the proportion of polymorphic nucleotide sites were estimated to be 0.0024 and 0.007, respectively. The mean number of insertional differences in this region between random pairs of chromosomes was 0.95. A sign...