The enzymes, cr-galactosidase, P-galactosidase, a-mannosidase, P-glucosidase, and p-acetylglucosaminidase, have been purified from the germinating seeds of Phaseolus vulgaris. All five enzymes have been simultaneously isolated in a highly active form. The pH optimum, K,,,, and energy of activation of each glycosidase for the reaction of hydrolysis of the appropriate P-nitrophenyl glycoside have...