Negative stain electron microscopy (EM) allows relatively simple and quick observation of macromolecules and macromolecular complexes through the use of contrast enhancing stain reagent. Although limited in resolution to a maximum of ~18 - 20 Å, negative stain EM is useful for a variety of biological problems and also provides a rapid means of assessing samples for cryo-electron microscopy (cry...

Gingivitis is an inflammatory destructive disease mainly caused by microbial plaques. Gram-positive and gram-negative bacilli or cocci have been isolated from dental plaque and gingivitis. This study was going to isolate and characterize the pathogenic bacteria from gingival of 100 patients with oral gingivitis. Enriched and specific media used for isolation of bacteria. Identification of isola...

The fine structure of resting and activated platelets was compared using two approaches novel to this dense cytoplasm. First, rapid lysis of platelets on carbon-coated grids was following by negative staining of the "cytoskeleton." Second, a brief, minimal fixation of platelets in plasma was coupled with partial lysis and examination of the unstained whole mounts at 200 kV. The results showed t...

Nuclear accumulation ofp53 protein Is associated with a poorer clinical outcome in breast cancer patients. Heat shock protein 70 (lssp7O) is a chaperone that binds to mutant p53 and consequently could regulate its accumulation or localization. The aims of this study were to determine if the prognostic significance ofp53 accumulation was dependent on the type ofantibody used for detection and wh...

Although sequencing provides the gold standard for identifying colorectal carcinoma with BRAF V600E mutation, immunohistochemistry (IHC) with the recently developed mouse monoclonal antibody VE1 for BRAF V600E protein has shown promise as a more widely available and rapid method. However, we identified anecdotal discordance between VE1 IHC and sequencing results and therefore analyzed VE1 stain...