Nerve growth factor (NGF) and laminin are important factors for neural development and regeneration. We examined the effects of increasing the local concentration and duration of action of NGF and laminin on peripheral nerve regeneration in the adult mouse sciatic nerve. A Silastic (silicone rubber) channel with intraluminal NGF solution was secured between transected nerve ends. The second cha...

These studies seek to determine whether the locomotory behavior of the extending tip of growing nerve fibers exhibits chemotaxis to nerve growth factor (NGF). Sensory neurons from dissociated dorsal root gangha of chick embryos were cultured within semisolid agar matrices containing concentration gradients of NGF which diffused from an adjacent source. A preferential orientation of approximatel...

Strategies of Schwann cell (SC) transplantation to regenerate the peripheral nerve injury involves many limitations. Stem cells can be used as alternative cell sources for differentiation into SCs. Given the high potential of neural crest-derived stem cells for the generation of multiple cell lineages, in this research, we considered whether olfactory ecto-mesenchymal stem cells (OE-MSCs) derive...

BACKGROUND For peripheral nerve regeneration, recent attentions have been paid to the nerve conduits made by tissue-engineering technique. Three major elements of tissue-engineering are cells, molecules, and scaffolds. METHODS In this study, the attachments of nerve cells, including Schwann cells, on the nerve conduit and the effects of both growth factor and adhesion molecule on these attach...

The cell surface receptor for beta-nerve growth factor was used as a probe to study the development of embryonic chick sensory ganglia. The ganglia were shown to lose their responsiveness to nerve growth factor in vitro between 14 and 16 days of embryonic age. This loss occurred by a decrease in the magnitude of the maximum biological response, not by a shifting of the response to higher concen...

OBJECTIVE To investigate the impacts of anti-nerve growth factor antibody on pain-related behaviors and expressions of μ-opioid receptor in spinal dorsal horn and dorsal root ganglia of rats with cancer-induced bone pain. METHODS The rats were randomly grouped and then injected with 10 μl of phosphate buffer saline or Walker256 tumor cells into the upper segment of left tibia. Thirteen days a...