Thanks to the changes in medicine, pharmacological treatment rapidly progresses into new fields. There is an emphasis on the development of treatment methods to eliminate underlying factors rather than to treat the symptoms of a disease. Therefore, research is increasingly utilizing knowledge from the field of genetics. Genetic mutation and deletion lead to many genetic disorders. Genetic disor...

Unlike the development of drugs based on small chemical entities there are no conventional regulatory toxicity studies established for DNA-based products. As the potential for insertional mutagenesis is of particular concern for gene therapy, we have investigated the mutagenicity of model non-viral DNA-based products at the HPRT locus in Chinese hamster V79 cells. Cultures were transfected with...

objective(s):  although several chemical and physical methods for gene delivery have been introduced, their cytotoxicity, non-specific immune responses and the lack of biodegradability remain the main issues. in this study, hydroxyapatite nanoparticles (nps) and 1,2-dioleoyl-sn-glycero-3-phosphoethanol​amine (dope)-modified hydroxyapatite nps was coated with antisense oligonucleotide of e6 mrna...