In mouse kidney, the conventional protein kinase C (PKC) isoenzyme alpha is expressed in glomeruli, the cortical collecting duct (intercalated cells only), and medullary collecting duct. To get insights on its function, PKC-alpha knockout (-/-) and wild-type (+/+) mice were studied. When provided free access to water, PKC-alpha -/- mice showed approximately 50% greater urine flow rate and lower...

Adrenomedullin and the natriuretic peptides exert vasodilator, natriuretic, and aldosterone-inhibitory actions, making augmentation of both systems potential therapeutic strategies in heart failure. Adrenomedullin and an endopeptidase inhibitor (SCH32615) were administered separately and in combination in 8 sheep with heart failure. Compared with the control condition, SCH32615 (5 mg bolus+1 mg...

Background: The ratio of urine protein to urine osmolality has recently been suggested as an accurate method to determine proteinuria.  Objective: We studied the correlation of urine protein to urine osmolality ratio with 24-hr urinary protein excretion. Methods: 150 children aged 0.11–17 years admitted to the Department of Pediatric Nephrology were included in this study. Early morning urine s...

Chronic inflammation contributes to the development and progression of chronic kidney disease (CKD). Identifying renal inflammation early is important. There are currently no specific markers of renal inflammation. Endothelin-1 (ET-1) is implicated in the pathogenesis of CKD. Thus, we investigated the impact of progressive renal dysfunction and renal inflammation on plasma and urinary ET-1 conc...

background: the ratio of urine protein to urine osmolality has recently been suggested as an accurate method to determine proteinuria.   objective: we studied the correlation of urine protein to urine osmolality ratio with 24-hr urinary protein excretion.   methods: 150 children aged 0.11–17 years admitted to the department of pediatric nephrology were included in this study. early morning urin...

BACKGROUND Urine is increasingly becoming an attractive biological fluid in clinical practice due to being an easily obtained, non-invasive sampling method, containing proteins and peptides. The aim of this study was to investigate eosinophiluria, urinary eosinophil cationic protein (uECP) and urinary IL-5 (uIL-5) in patients with Lupus Nephritis. METHODS Seventy-four patients with SLE-20 wit...

Background. Despite enzyme replacement therapy, Fabry nephropathy still progresses. Podocyturia is an irreversible event that antedates proteinuria and leads to chronic renal failure. We evaluated a potential mechanism of podocyte detachment via the expression of the urokinase-type Plasminogen Activator Receptor (uPAR) in urinary podocytes of Fabry patients. Methods. This is a cross-sectional s...

Some of the variables in the Folin method for measurement of urinary creatine and creatinine were studied. The effects of varying the picric acid concentration, volume of solution, and heating time on the conversionof creatine to creatinine were investigated. A study was also made of the results obtained in the chromogenic reaction by varying the concentration of picric acid and sodium hydroxid...

OBJECTIVE To study the impact of thyroxine (T4) withdrawal on serum osteoprotegerin concentrations in women, using a healthy euthyroid control group matched for age and postmenopausal status as reference. SUBJECTS AND DESIGN Nineteen women with differentiated thyroid carcinoma were studied the last day on T4 suppressive treatment, 4-7 days after withdrawal and the day before whole body scanni...

Two hundred thirty-three randomly selected families provided a population for studying the effects of familial relationships, age, diet, body weight, and urinary electrolyte excretion on blood pressure. There was a strong familial component for urinary sodium, potassium, and creatinine excretion and for systolic blood pressure. In individuals, age, heart rate, and body weight were independently...