Solitary bees and honey bees from a neighbouring apiary were screened for a broad set of putative pathogens including protists, fungi, spiroplasmas and viruses. Most sampled bees appeared to be infected with multiple parasites. Interestingly, viruses exclusively known from honey bees such as Apis mellifera Filamentous Virus and Varroa destructor Macula-like Virus were also discovered in solitar...

Comparative small subunit rDNA sequence analyses, indicate that Ovavesicula popilliae, a microsporidian parasite of the Japanese beetle, Popillia japonica, represents a distant sister group to Paranosema and Antonospora. These three genera represent a second major group (the Nosema/Vairimorpha clade representing the first) of Microsopridia which infect terrestrial insects, suggesting independen...

This study is focused on the investigation of honey bee samples for presence two most common and widely distributed parasites. In a two-year period during 2020-2021, 185 were tested. All examined by morphological light microscopic methods. The obtained results showed that 32.43% infested with Varroa destructor. degree infection in bees ranged from 0.5% to 60%. Spores Nosema spp. established 25....

A technique for the optimum preparation of microsporidia for scanning electron microscopy is described. Microsporidian-infected tissues were subjected to a triple fixation procedure followed by slow dehydration and critical point drying. Fracturing of the tissue to reveal details of both host and parasite was delayed until immediately before coating of specimens with gold; the exposed parasites...