Background: The most common severe symptoms of coronavirus disease 2019 (COVID-19) are breathing difficulty and respiratory failure. Unfortunately, mortality COVID-19 patients with difficulties is high. Clinical finding diagnosis: This report describes the case a woman in her seventies, who could not taste anything except metallic ...

Background: Children born preterm (before 37 weeks of gestation) are at risk for several adverse childhood outcomes. Parent priorities research into these outcomes, and preferences receiving information about risks, have not previously been established. Here we report the results an online survey designed to understand parent their outcomes. ...

Background: Digital interventions have the potential to reduce alcohol consumption, although evidence on effectiveness of apps is lacking. Drink Less a popular, evidence-informed app with good usability, putting it in strong position be improved upon prior conducting confirmatory evaluation. This paper describes process refining improve its u...

This study sought to determine the effect of fermented medicinal herbs (FMH), i.e. cutchery (Kaempferia galanga), turmeric (Curcuma longa) and curcuma xanthorrhiza) in combination with molasses probiotic drink (Yakult), administered orally on hematological physiological profile of...

Background: Tetanus remains common in many low- and middle-income countries (LMICs) yet the evidence base guiding management of this disease is extremely limited, particularly with respect to contemporary options. Sharing knowledge about practice may facilitate improvement outcomes elsewhere. Methods: We describe clinical intervent...

Background: There is limited data on the burden of mental disorders among ‘healthy’ children in Africa. We examined prevalence and correlates neurocognitive psychiatric schoolchildren Uganda. Methods: This cross-sectional study enrolled 322 aged 5-17years Wakiso, Uganda. assessed for impairment using Kaufmann-Assessment-Battery, (m...

The NS3 protein of hepatitis C virus (HCV) has a serine protease activity in its N-terminal region, which plays a crucial role in virus replication. This region has also been reported to interact not only with its viral cofactor NS4A, but also with a number of host-cell proteins, which suggests a multifunctional feature of NS3. By means of yeast two-hybrid screening using an N-terminal region o...

The NS2B-NS3(pro) polyprotein segment from the dengue virus serotype 2 strain 16681 was purified from overexpressing E. coli by metal chelate affinity chromatography and gel filtration. Enzymatic activity of the refolded NS2B-NS3(pro) protease complex was determined in vitro with dansyl-labeled peptide substrates, based upon native dengue virus type 2 cleavage sites. The 12mer substrate peptide...

Antiviral immunity requires recognition of viral pathogens and activation of cytotoxic and Th cells by innate immune cells. In this study, we demonstrate that hepatitis C virus (HCV) core and nonstructural protein 3 (NS3), but not envelope 2 proteins (E2), activate monocytes and myeloid dendritic cells (DCs) and partially reproduce abnormalities found in chronic HCV infection. HCV core or NS3 (...

The hepatitis C virus (HCV) nonstructural 3 protein (NS3) contains at least two domains associated with multiple enzymatic activities; a serine protease activity resides in the N-terminal one-third of the protein, whereas RNA helicase activity and RNA-stimulated nucleoside triphosphatase activity are associated with the C-terminal portion. To study the possible mutual influence of these enzymat...