نتایج جستجو برای: nuclear reprogramming

تعداد نتایج: 249719  

2012
María-Teresa Solís María Rodríguez-Serrano Mónica Meijón María-Jesús Cañal Alejandro Cifuentes María C. Risueño Pilar S. Testillano

Stress-induced plant cell reprogramming involves changes in global genome organization, being the epigenetic modifications key factors in the regulation of genome flexibility. DNA methylation, accomplished by DNA methyltransferases, constitutes a prominent epigenetic modification of the chromatin fibre which is locked in a transcriptionally inactive conformation. Changes in DNA methylation acco...

Journal: :The EMBO journal 2016
Ester Perales-Clemente Alexandra N Cook Jared M Evans Samantha Roellinger Frank Secreto Valentina Emmanuele Devin Oglesbee Vamsi K Mootha Michio Hirano Eric A Schon Andre Terzic Timothy J Nelson

Functional variability among human clones of induced pluripotent stem cells (hiPSCs) remains a limitation in assembling high-quality biorepositories. Beyond inter-person variability, the root cause of intra-person variability remains unknown. Mitochondria guide the required transition from oxidative to glycolytic metabolism in nuclear reprogramming. Moreover, mitochondria have their own genome ...

2015
Osamu Hashizume Sakiko Ohnishi Takayuki Mito Akinori Shimizu Kaori Ishikawa Kazuto Nakada Manabu Soda Hiroyuki Mano Sumie Togayachi Hiroyuki Miyoshi Keisuke Okita Jun-Ichi Hayashi

Age-associated accumulation of somatic mutations in mitochondrial DNA (mtDNA) has been proposed to be responsible for the age-associated mitochondrial respiration defects found in elderly human subjects. We carried out reprogramming of human fibroblast lines derived from elderly subjects by generating their induced pluripotent stem cells (iPSCs), and examined another possibility, namely that th...

2011
Rosa M. Marión Gunnar Schotta Sagrario Ortega Maria A. Blasco

Reprogramming of adult differentiated cells to induced pluripotent stem cells (iPS) cells has been achieved by over-expression of specific transcription factors. Nuclear reprogramming induces a series of profound changes at the telomeres of the parental differentiated cells, including a telomerase-dependent telomere elongation and the remodeling of telomeric chromatin. In particular, iPS cells ...

Journal: :Reproduction 2016
Bingteng Xie Heng Zhang Renyue Wei Qiannan Li Xiaogang Weng Qingran Kong Zhonghua Liu

Aberrant epigenetic reprogramming is the main obstacle to the development of somatic cell nuclear transfer (SCNT) embryos and the generation of induced pluripotent stem (iPS) cells, which results in the low reprogramming efficiencies of SCNT and iPS. Histone H3 lysine 27 trimethylation (H3K27me3), as a repressive epigenetic mark, plays important roles in mammalian development and iPS induction....

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