How nuclear shape correlates with nuclear movements during the cell cycle is poorly understood. We investigated changes in nuclear morphology during nuclear migration in budding yeast. In preanaphase cells, nuclear protrusions (nucleopodia [NP]) extend into the bud, preceding insertion of chromosomes into the bud neck. Surprisingly, formation of nucleopodia did not depend on the established nuc...

In many organisms, homolog pairing and synapsis at meiotic prophase depend on interactions between chromosomes and the nuclear membrane. Male Drosophila lack synapsis, but nonetheless, their chromosomes closely associate with the nuclear periphery at prophase I. To explore the functional significance of this association, we characterize mutations in nuclear blebber (nbl), a gene required for bo...

Changes in the shape of the nuclear lamina are exhibited in senescent cells, as well as in cells expressing mutations in lamina genes. To identify cells with defects in the nuclear lamina we developed an imaging method that quantifies the intensity and curvature of the nuclear lamina. We show that this method accurately describes changes in the nuclear lamina. Spatial changes in nuclear lamina ...

in this work, we report synthesis of silver nanoplates by a simple reduction process of silver nitrate in the presence of polyvinyl alcohol (pva) and n,n'-dimethyl formamide (dmf). the characterization of the samples were carried out using x-ray diffraction (xrd), transmission-electron microscopy (tem) and uv-vis spectroscopy. absorption spectra of the nanoplates in comparison with that of...

A phase space coalescence description based on the Wigner-function method for cluster formation in relativistic nucleus-nucleus collisions is presented. The momentum distributions of nuclear clusters d,t and He are predicted for central Au(11.6AGeV)Au and Si(14.6AGeV)Si reactions in the framework of the RQMD transport approach. Transverse expansion leads to a strong shoulderarm shape and differ...

Cellular organelles have characteristic morphologies that arise as a result of different local membrane curvatures. A striking example is the endoplasmic reticulum (ER), which consists of ER tubules with high curvature in cross-section, peripheral ER sheets with little curvature except at their edges and the nuclear envelope with low curvature except where the nuclear pores are inserted. The ER...

Mechanical stresses on the myocyte nucleus have been associated with several diseases and potentially transduce mechanical stimuli into cellular responses. Although a number of physical links between the nuclear envelope and cytoplasmic filaments have been identified, previous studies have focused on the mechanical properties of individual components of the nucleus, such as the nuclear envelope...

The nucleus is one of the most prominent cellular organelles, yet surprisingly little is known about how it is formed, what determines its shape and what defines its size. As the nuclear envelope (NE) disassembles in each and every cell cycle in metazoans, the process of rebuilding the nucleus is crucial for proper development and cell proliferation. In this Commentary, we summarize what is kno...

Chao, P G; Bulinski, J C; Ateshian, G A; +Hung, C T Columbia University, New York, NY [email protected] INTRODUCTION Cartilage compression changes cell shape, volume and hydrostatic and osmotic pressure of chondrocytes in situ [1,2]. For example, changes in the osmotic environment of cells have been shown to modulate chondrocyte biosynthesis, however, the mechanosensing mechanisms are not well...

Changes in the shape and structural organization of the cell nucleus occur during many fundamental processes including development, differentiation and aging. In many of these processes, the cell responds to physical forces by altering gene expression within the nucleus. How the nucleus itself senses and responds to such mechanical cues is not well understood. In addition to these external forc...