We describe an enzyme-linked immunoassay for measuring human placental lactogen (HPL) in serum. After suitable dilution, sera are pipetted into the wells of polyvinyl microtitre plates previously coated with anti-HPL. After incubation the sera are decanted, and replaced by a conjugate of horseradish peroxidase and anti-HPL. An amount of enzyme proportional to the concentration of HPL in the ser...

Benzimidazole derivatives are known to represent a class of medicinally important compounds which are extensively used in drug design and catalysis. A series of 2-substituted benzimidazole derivatives 10a-i was herein synthesized from the reaction of o-phenylenediamine with some amino acids using ameliorable pathway. The chemical structures of the synthesized compounds were confirmed by IR, UV,...

LOOD OXIDASES HAVE BEEN DESCRIBED and discussed since 1912 based on tedious, elaborate means for the determination of their activity. The physiologic significance of oxidase activity has been implicitly associated with the blue copper protein of plasma designated as ceruloplasmin (1, 2, 3, 4, 5, 6). There appears to be a need for a simple, rapid procedure for the determination of ceruloplasmin ...

The effects of kaempferol on the oxidative and phosphorylative properties of plant mitochondria from potato tubers and etiolated mung bean (Phaseolus aureus Roxb.) hypocotyls were investigated. Kaempferol inhibited the state 3 oxidation rate of malate, NADH, and succinate, but was without effect on the ascorbate-tetramethyl p-phenylenediamine oxidation rate. The inhibition was almost the same w...

Allergic Contact Dermatitis to p-phenylenediamine and Some of its Reaction Products E. Young*, E. Zimerson, C. Svedman, M. Bruze Department of Occupational and Environmental Dermatology, Skåne University Hospital, Lund University, Malmö, Sweden *Corresponding author: Dr. Ewa Young Department of Occupational and Environmental Dermatology Skåne University Hospital 205 02 Malmö, Sweden, Tel: +46-4...

Economic and environmentally friendly bio-mimetic one-pot three and four-component Knoevenagel-hydrogenation (K-H), five-component Knoevenagel-hydrogenation-alkylation (K-H-A) and six-component Knoevenagel-hydrogenation-alkylation-Huisgen cycloaddition (K-H-A-HC) reactions of aldehydes, CH-acids, o-phenylenediamine, alkyl halides and azides using proline, proline-metal carbonate and proline-met...

The effects of N-methylphenazonium methosulfate (PMS) have been compared with three other phenazine dyes and N, N, N’, N’-tetramethyl-&phenylenediamine (TMPD). All accelerate the recovery of the initial absorbance changes in Rhodospirillum rubrum extracts induced by a lO+ec flash, although only N-ethylphenazonium ethosulfate and TMPD stimulate photophosphorylation to the same extent as PMS. N-E...

BACKGROUND A nation-wide Norwegian Patch Test Registry (NOLAR) was established in 2005 as a collaboration between six dermatology departments. International, multi-centre studies have documented great variability in the frequency of positive patch test reactions, considered as mainly due to heterogeneity of test populations. OBJECTIVES To analyse the variability of positive test reactions by ...

Recently, marked oxygen dependence of respiration by isolated mitochondria after exposure to prolonged hypoxia has been described. Because mitochondrial oxygen-dependent respiration could significantly influence oxygen consumption during critical illness, we sought to confirm the oxygen-dependent behavior of mitochondria. We hypothesized that mitochondria isolated during sepsis would exhibit in...

Optimum reaction conditions were evaluated for assay of serum ceruloplasmm by measurement of its p-phenylenediamine oxidase activity. The pH optima of p-phenylenediamine oxidase activities of human and rat ceruloplasmins were 5.45 and 5.2, respectively. The p-phenylenediamine oxidase activity of rat ceruloplasmin was markedly inhibited by phosphate (0.1 mol/Iiter), that of human ceruloplasmin o...