Oligonucleotide probes specific for simple tandem repeat sequences produce individual specific DNA fingerprints in man and all animal species so far. In the present investigation (r)-p labeled oligonucleotide probes (GTG)5, (GT)8 and (GGAT)4 were used as multilocus probes to detect hypervariable microsatellites in the Indian dromedary camel population. Oligo probes were radiolabelled and hybrid...

Single molecule FISH (smFISH) allows studying transcription and RNA localization by imaging individual mRNAs in single cells. We present smiFISH (single molecule inexpensive FISH), an easy to use and flexible RNA visualization and quantification approach that uses unlabelled primary probes and a fluorescently labelled secondary detector oligonucleotide. The gene-specific probes are unlabelled a...

Fluorescent in situ hybridization (FISH) using rRNA-specific oligonucleotide probes has emerged as a popular technique for identifying individual microbial cells. In natural samples, however, the signal derived from fluor-labeled oligonucleotide probes often is undetectable above background fluorescence in many cells. To circumvent this difficulty, we applied fluorochrome-labeled polyribonucleo...

Gene microarrays provide a powerful functional genomics technology which permits the expression profiling of tens of thousands of genes in parallel. The basic idea of their functioning is based on the sequence specificity of probe–target interactions combined with fluorescence detection. In reality, this straightforward principle is opposed by the complexity of the experimental system due to im...