نتایج جستجو برای: one stepone tube pcr
تعداد نتایج: 2220314 فیلتر نتایج به سال:
Background and Aim: Brucellosis is a zoonotic disease and is one of the major health problems in our country. Considering its nonspecific symptoms, diagnosis of this disease is often made by laboratory methods. The purpose of the present study was to compare the sensitivity and specificity of ELISA and a relatively new modified Rose Bengal test in the diagnosis of brucellosis. Material and Meth...
AIMS To evaluate the usefulness of a single-tube nested polymerase chain reaction (PCR) assay in the diagnosis of tuberculosis in 1497 pulmonary and 536 extrapulmonary specimens. METHODS A single-tube nested PCR, utilising two sets of primers with different melting temperatures (88 degrees C for external primers; 70 degrees C for internal primers) to augment sensitivity and specificity withou...
blood collection in heparin tubes for cytogenetic, and ethylenediaminetetraacetic acid (edta) tubes for molecular genetics applications respectively, are routine practices everywhere. if blood samples are required for cytogenetics as well as dna work, two samples from each animal are usually collected, which leads to wastage of time and money. the present study tried to explore the possibilitie...
BACKGROUND High-resolution melting of PCR amplicons with the DNA dye LCGreen I was recently introduced as a homogeneous, closed-tube method of genotyping that does not require probes or real-time PCR. We adapted this system to genotype single-nucleotide polymorphisms (SNPs) after rapid-cycle PCR (12 min) of small amplicons (</=50 bp). METHODS Engineered plasmids were used to study all possibl...
OBJECTIVE To verify the incidence of the G679A mutation in exon 2 of the gene inhibin alpha (INHA), in women with secondary amenorrhea and diagnosis of premature ovarian insufficiency, and in controls. METHODS A 5mL sample of peripheral blood was collected from all study participants in an EDTA tube and was used for DNA extraction. For the patient group, 5mL of blood were also collected in a ...
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