In spite of great efforts, the mechanisms for postovulatory oocyte aging are not fully understood. Although our previous work showed that the FasL/Fas signaling facilitated oocyte aging, the intra-oocyte signaling pathways are unknown. Furthermore, the mechanisms by which oxidative stress facilitates oocyte aging and the causal relationship between Ca2+ rises and caspase-3 activation and betwee...

Improved methods are needed to reliably and accurately evaluate oocyte quality prior to fertilization and transfer into the woman of human embryos created through in vitro fertilization (IVF). All oocytes that are retrieved and matured in culture are exposed to sperm with little in the way of evaluating the oocyte quality. Furthermore, embryos created through IVF are currently evaluated for dev...

The management of IT carve-out projects is very challenging due to the strict time frame, the severe contractual penalties, the huge number of stakeholders and the various as well as unique IT tasks that have to be conducted. Up to now, there is no instrument for evaluating the readiness of the IT for a carve-out and also not for managing such a project. In order to address this, we develop a m...

Background: The cryopreservation of human oocyte would make significant contribution to fertility treatments. It could also provide on alternative to embryo preservation for avoidance ethical problems. Embryo cryopreservation is now a successful procedure, but oocyte cryopreservation has poorer results. Materials and Methods: For retrieval of oocyte NMRI mouse were used. after administration of...

Fatty acid oxidation is an important energy source for the oocyte; however, little is known about how this metabolic pathway is regulated in cumulus-oocyte complexes. Analysis of genes involved in fatty acid oxidation showed that many are regulated by the luteinizing hormone surge during in vivo maturation, including acyl-CoA synthetases, carnitine transporters, acyl-CoA dehydrogenases and acet...

introduction: semen parameters and specially sperm morphology is used for the assessment of semen sample and for the prediction of the fertilization. however there is semen samples with normal sperm morphology but with hidden sperm chromatin anomalies. since sperm morphology and chromatin anomalies reduce the fertilization rat independently, the purpose of this study is to consider the relation...

The reproductive characteristics of an endemic labeonine fish, Garra tibetana, were investigated by examining 778 individuals collected monthly, from December 2015 to November 2016, in the lower Yarlung Tsangpo River, Tibet, China. Results showed that females predominated overall population with a sex ratio 1:0.73, while for mature was 1:1. Standard length at first maturity estimated as 82.4 mm...

The mammalian oocyte develops within a complex of somatic cells known as a follicle, within which signals from the somatic cells regulate the oocyte, and signals from the oocyte regulate the somatic cells. Because isolation of the oocyte from the follicle disrupts these communication pathways, oocyte physiology is best studied within an intact follicle. Here we describe methods for quantitative...

The oocyte is a highly differentiated cell. It makes organelles specialized to its unique functions and progresses through a series of developmental stages to acquire a fertilization competent phenotype. This review will integrate the biology of the oocyte with what is known about oocyte-specific gene regulation and transcription factors involved in oocyte development. We propose that oogenesis...