924 BioTechniques Vol. 34, No. 5 (2003) inates the residual ribonuclease activity, although the effect of this treatment on the subsequent experiments should be checked. An inhibitor of RNase, such as Rnasin, can also be added to further enzymatic reactions. This study has described a degrading activity in RNA preparations from ovine, bovine, and porcine brain and ovine liver. Further experimen...

The convulsant opiate thebaine, an intermediate of morphine biosynthesis, was purified from bovine brain to homogeneity by gel filtration and high-performance liquid chromatography (HPLC) monitored by a radioimmunoassay. The immunoreactive material behaved identically to standard thebaine in two HPLC systems and was confirmed to be thebaine by combined gas chromatography/mass spectrometry. To o...

An in vitro system was used to study the regulation of glucose and acetate utilization by bovine and ovine adipose tissue (AT). Adult nonlactating, nonpregnant cows ( n = 5 ) and ewes ( n = 5 were given limited amounts of feed for 8 to 10 d, then overfed for 10 (ewes) or 21 (cows) d. Perirenal AT explants were incubated for 7 d. The effect of 2 mu/ mL of insulin on glucose utilization was signi...

Ce chapitre décrit l’évolution des marchés et présente les projections à moyen terme relatives aux mondiaux de la viande sur période 2023-32. Il passe en revue évolutions prévues matière consommation, production, d’échanges prix pour bovine, porcine, volaille ovine. s’achève par un examen principaux risques incertitudes susceptibles d’avoir une incidence dans dix prochaines années.

this study was conducted on the atrioventricular node (avn) and atrioventricular bundle (avb) of fivefour-month-old ovine fetuses. the histological structure of these components was studied by routinehistological techniques and use of specific staining methods. the avn was caudally located adjacent to theroot of the aorta. it was almost spherical in shape and consisted of twisty cells. the node...

background: this study was aimed to assess the effects of angiotensin ii (ang ii) supplementation to the in vitro maturation (ivm) and in vitro culture (ivc) media of vitrified-warmed ovine oocytes on their developmental competence and expression of na+/k+/atpase in resulting embryos. methods: the slaughterhouse-derived immature oocytes (n=1069) were randomly distributed into four experimental ...

Background: No validated and standardized animal models of pulmonary air leakage (PAL) exist for testing aerostatic efficacy lung sealants. Lack negative control groups in published studies intrinsic sealing mechanisms healthy lungs might contribute to a translational gap, leading poor clinical results. This study aims address the impact on validity PAL models, investigate conditions required a...

BACKGROUND AND OBJECTIVE Renal denervation has recently become of great interest as a potential treatment for resistant hypertension. Denervation techniques using radio frequency (RF) or ultrasound energy sources have already been explored in literature. In this study, we investigate the use of lasers as a potential energy source for renal denervation. In vitro studies are performed in porcine/...

Prion diseases are characterized by a conformational change in the normal host protein PrPC. While the majority of mature PrPC is tethered to the plasma membrane by a glycosylphosphatidylinositol anchor, topological variants of this protein can arise during its biosynthesis. Here we have generated Drosophila transgenic for cytosolic ovine PrP in order to investigate its toxic potential in flies...