OBJECTIVE Enhanced oxidative stress is involved in mediating the endothelial dysfunction associated with hypertension. The aim of this study was to investigate the relative contributions of pro-oxidant and anti-oxidant enzymes to the pathogenesis of endothelial dysfunction in genetic hypertension. METHODS Dilator responses to endothelium-dependent and endothelium-independent agents such as ac...

In Traditional Ayurvedic system of treatment Tragia involucrata Linn. is intended to use for diabetic patients has been screened for antioxidant activity. For antioxidant studies, aqueous ethanolic extract of Tragia involucrata (AEETI) was administered orally for 28 days at a dose of 250 and 500 mg/kg body weight to StreptozotocinNicotinamide induced Wistar rats. All the animals were sacrificed...

Drought stress is the most common environmental stress that can significantly influence crop productivity. In this study, morphological and biochemical properties of 17 genotypes of common bean were evaluated under different levels of drought stress and the most sensitive and tolerant genotypes were identified using multivariate analysis. The results indicated that morphological and biochemical...

Electromagnetic waves affect living organisms and it is of great interest for wide interaction of new sources with a diversity of frequencies and powers to life of people. In the last few years, many authors have proposed that the biological effect of electromagnetic fields in both the high-frequency and low-frequency ranges are connected with oxidative processes in tissues. Studying the change...

Electromagnetic waves affect living organisms and it is of great interest for wide interaction of new sources with a diversity of frequencies and powers to life of people. In the last few years, many authors have proposed that the biological effect of electromagnetic fields in both the high-frequency and low-frequency ranges are connected with oxidative processes in tissues. Studying the change...

Coupled enzyme assays are described for measuring inorganic phosphates, organic phosphates and phosphate-liberating enzymes in biological material. The assays all determine Pi by its reaction with inosine, catalysed by nucleoside phosphorylase; this yields ribose 1-phosphate and hypoxanthine. The hypoxanthine is oxidized to uric acid by xanthine oxidase, and may be measured either by the absorb...

Asthma is a chronic inflammatory airway disease characterized by allergen-induced airway hyperresponsiveness, airway inflammation, and remodeling. Nitric oxide (NO) derived from constitutive and inducible enzymes affects many aspects of asthma physiopathology. Animal in vivo studies have indicated that inhibition of iNOS may play a central role in the modulation of these features, particularly ...

Atherosclerotic vascular dysfunction is a chronic inflammatory process that spreads from the fatty streak and foam cells through lesion progression. Therefore, its early diagnosis and prevention is unfeasible. Reactive oxygen species (ROS) play important roles in the pathogenesis of atherosclerotic vascular disease. Intracellular redox status is tightly regulated by oxidant and antioxidant syst...

Hepatic diseases are serious health issues as various pharmaceuticals, xenobiotics, environmental/chemical pollutants, some natural products and dietary supplements when used in overdose have tendency to cause hepatotoxicity. Present study was aimed at investigating hepatoprotective activity of aqueous methanolic extract of Euphorbia prostrata against paracetamol and carbon tetrachloride induce...

A set of bifunctional oxidase-peroxidases has been prepared by fusing four distinct oxidases to a peroxidase. Although such fusion enzymes have not been observed in nature, they could be expressed and purified in good yields. Characterization revealed that the artificial enzymes retained the capability to bind the two required cofactors and were catalytically active as oxidase and peroxidase. P...