The distribution of cellular unesterified cholesterol was studied in fibroblasts, which had been depleted of plasma membrane sphingomyelin by exposure to exogenous sphingomyelinase. This treatment has previously been shown to induce an increase in cholesterol esterification, a decrease in the biosynthesis of cholesterol, and a decreased susceptibility of cell cholesterol to oxidation with chole...

The aim of the present study was to elucidate the intracellular mechanisms that cause neuronal cell death following exposure to excitatory neurotransmitter‑induced neurotoxicity, neurotoxins and oxidative stress. Human SH‑SY5Y neuroblastoma cells were exposed to various stimuli, including glutamate, 6‑hydroxydopamine (6‑OHDA), and glucose oxidase, and cell viability was determined by MTT assay....

The recognition of Cryptococcus neoformans in Gram-stained smears of purulent exudates may be hampered by the presence of the large gelatinous capsule which apparently prevents definitive staining of the yeast-like cells. In such stained preparations, C. neoformans may appear either as round cells with gram-positive granular inclusions impressed upon a pale lavender cytoplasmic background or as...

Eight strains of an unclassified, oxidase-negative, lactose-fermenting, urease-producing, gram-negative bacillus were isolated from clinical material. With the exception of urease production, the biochemical characteristics of these organisms resembled those of Escherichia coli.

Despite the efforts made to identify Pseudomonas aeruginosa by saccharolytic methods, this gram-negative microorganism remains predominately a proteolytic bacillus with weak saccharolytic activity. Pigmentation, growth temperature, pellicle formation and other minor characteristics are unsatisfactory laboratory diagnostic criteria (Gaby, 1946, 1955; Gaby and Free, 1953). Needless to say it is h...

The results of this study of the effect of temperature on the respiratory mechanism of five stenothermophilic bacteria may be summarized as follows:- 1. The respiratory mechanism and its various components of the stenothermophilic bacteria were found to function at temperatures below the minimum temperature for growth of these organisms. In every case the rates of the individual reactions invol...

Various protolytic residues in subunit I of aa3-600 quinol oxidase of the aerobic Gram-positive Bacillus subtilis were mutagenized to nonpolar residues. Two of the mutations, Y284F and K304L, impaired the bioenergetic function of the microorganism. The Y284F mutation suppressed the electron-transfer activity of quinol oxidase and altered its interaction with CO and H2O2, thus showing destructio...

275 chorea occurs when the function of the corpus striatum is disrupted, the specific abnormality in Sydenham's chorea is unknown. The response to valproic acid suggests that the defect affects the GABA neurotransmitter system. Possibly the difference in effectiveness of valproic acid between Sydenham's chorea and Huntington's chorea reflects the degree of severity of the pathologic process or,...

Background: To diagnose the LRTIs (lower respiratory tract infections) in microbiological laboratory, most frequentlyemployed method is microscopic examination of expectorated sputum samples. These samples are generallycontaminated with normal flora that resident oropharynx. manage and lower infections,sputum collection, culture, microscopy vital.Aim: The present study was conducted to analyse ...

we report a catalase-negative staphylococcus aureus isolated from a 56-year-old male diabetic patient with foot ulcer who attended our surgery ward. species identification was confirmed by gram staining, standard biochemical tests and pcr amplification of the nuc and fem genes. antibiotic susceptibility showed that the strain was sensitive to imepenem, chloramphenicol, amoxicillin, vancomycin a...