Measurement of mitochondrial function in skeletal muscle is a vital tool for understanding regulation of cellular bioenergetics. Currently, a number of different experimental approaches are employed to quantify mitochondrial function, with each involving either mechanically or chemically induced disruption of cellular membranes. Here, we describe a novel approach that allows for the quantificat...

There is a considerable discrepancy between oxygen supply and demand in the liver because hepatic oxygen consumption is relatively high but about 70% of the hepatic blood supply is poorly oxygenated portal vein blood derived from the gastrointestinal tract and spleen. Oxygen is delivered to hepatocytes by blood flowing from a terminal branch of the portal vein to a central venule via sinusoids,...

See article by Kiriazis and Gibbs [1] (pages 111 –119) two parts, activation and basal oxygen consumption. The in this issue. first depends on excitation–contraction coupling and is proportional to cardiac contractility. For the quiescent muscle the PVA and the oxygen cost of activation both are zero. The remaining part results from all other processes 1. Work output and efficiency of the heart...