Abstract Background The SARS-CoV-2 pandemic has had an unprecedented impact on healthcare systems, and cancer patients were amongst the most vulnerable. CONTACT is a national multidisciplinary study assessing of upon diagnostic treatment pathways among with pancreatic ductal adenocarcinoma (PDAC). Methods A novel, mixed prospective retrospective design, case identification both cohorts, trainee...

1 1 2 3 4 5 6 Distinctive receptive field and physiological properties of a wide-field amacrine cell in the macaque 7 monkey retina 8 9 10 Michael B. Manookin, Christian Puller, Fred Rieke, Jay Neitz, Maureen Neitz 11 Department of Ophthalmology, Physiology and Biophysics Department, HHMI, University of 12 Washington, Seattle, WA 98109 13 14 Corresponding authors: Michael B. Manookin (manookin@...

Transcripts of both mitochondrial and nuclear DNA replication genes accumulate periodically during the cell cycle in Crithidia fasciculata. An octameric consensus sequence with a conserved hexameric core was found previously to be required for cycling of the TOP2 transcript, encoding the mitochondrial DNA topoisomerase. We show here that the rate of synthesis of the p51 protein, the large subun...

The recent finding that the E1 glycoproteins of murine coronaviruses contain only O-linked oligosaccharides suggested that this unusual modification might be a distinguishing feature of coronaviruses and might play an essential role in the life cycle of this family of viruses. To examine these possibilities, we analyzed the oligosaccharide moieties of the membrane proteins of the avian coronavi...

In retroviruses, such as human immunodeficiency virus type 1 (HIV-1), the reverse transcriptase (RT) copies single-stranded viral RNA into complementary DNA, which is then used as a template for synthesis of the second DNA strand. The resulting double-stranded DNA is integrated into the host genome. How RT translocates on the different templates is the subject of this study. We have developed a...

Post-streptococcal autoimmunity affects millions worldwide, targeting multiple organs including the heart, brain, and kidneys. To explore the post-streptococcal autoimmunity spectrum, we used western blot analyses, to screen 310 sera from healthy subjects with (33%) and without (67%) markers of recent streptococcal infections [anti-Streptolysin O (ASLO) or anti-DNAse B (ADB)]. A 58 KDa protein,...

Six overlapping viral RNAs are synthesized in cells infected with the avian coronavirus infectious bronchitis virus (IBV). These RNAs contain a 3'-coterminal nested sequence set and were assumed to be viral mRNAs. The seven major IBV virion proteins are all produced by processing of three polypeptides of ca. 23, 51, and 115 kilodaltons. These are the core polypeptides of the small membrane prot...

A key step in proliferation of retroviruses is the conversion of their RNA genome to double-stranded DNA, a process catalysed by multifunctional reverse transcriptases (RTs). Dimeric and monomeric RTs have been described, the latter exemplified by the enzyme of Moloney murine leukaemia virus. However, structural information is lacking that describes the substrate binding mechanism for a monomer...