Polyhydroxyalkanoates (PHAs) are intracellular reserve material stored by gram-negative bacteria under nutrient-limited condition. PHAs are utilized in biodegradable plastics (bio-plastics) synthesis due to their similarity with conventional synthetic plastic. In the present study, the effect of addition of saturated and unsaturated fatty acids (palmitic acid, stearic acid, oleic acid and linol...

Desaturation of [1-r4C]stearic acid, [lJ4C]palmitic acid, and [l-14C]stearoyl-CoA was measured in homogenates of lo-day-old rat brain. Desaturation was maximal with stearoyl-CoA at pH 6.0 to 6.6. With free fatty acids, monoene formation was maximal at pH 7.2 to 7.6; stearic acid was more actively desaturated than palmitic acid. Activity with stearic acid or stearoyl-CoA was linear with protein ...

Desaturation of [1-r4C]stearic acid, [lJ4C]palmitic acid, and [l-14C]stearoyl-CoA was measured in homogenates of lo-day-old rat brain. Desaturation was maximal with stearoyl-CoA at pH 6.0 to 6.6. With free fatty acids, monoene formation was maximal at pH 7.2 to 7.6; stearic acid was more actively desaturated than palmitic acid. Activity with stearic acid or stearoyl-CoA was linear with protein ...

The lipid composition and metabolism of isolated guinea pig megakaryocyte subgroups at various stages of maturation were investigated. Three groups were studied: 1) 67% of megakaryocytes in Group A were immature; 2) Group B was heterogeneous and contained both immature and mature subgroups of megakaryocytes; 3) 92% of megakaryocytes in Group C were mature. Lipid composition was determined by th...

Biodegradable polymeric nanoparticles are widely recognized as efficacious drug delivery vehicles, yet the rational engineering of nanoparticle surfaces in order to improve biodistribution, reduce clearance, and/or improve targeting remains a significant challenge. We have previously demonstrated that an amphiphilic conjugate of avidin and palmitic acid can be used to modify poly(lactic-co-glyc...

In this issue of the Journal there are 2 studies that tested the cholesterol-raising effects of palmitic acid–rich palmolein and that reach apparently different conclusions. Tholstrup et al (1) found in 32 participants that palm oil elevated LDL cholesterol significantly by 0.22 mmol/L (P , 0.001) compared with olive oil, whereas Voon et al (2) found the difference between palm oil and olive oi...

Circulating triacylglycerol (TG) arises mainly from dietary fat. However, little is known about the entry of dietary fat into the major TG pool, very low-density lipoprotein (VLDL) TG. We used a novel method to study the specific incorporation of dietary fatty acids into postprandial VLDL TG in humans. Eight healthy volunteers (age 25.4 +/- 2.2 years, body mass index 22.1 +/- 2.3 kg/m2) were fe...

Background: This study aimed at determining the appropriate method for dewatering and drying biomass and selecting a suitable organic solvent for lipid extraction. Methods: Nannochloropsis Oculata was cultured in Gillard F/2 medium and after reaching the end of the stationary growth phase, algal biomass was separated from aqueous by centrifuge and dried through three methods: Oven, Air-dried an...