I n tr o d u c tio n T E cfi'ccls o f the systemic adminis­ tration o f crude papain to young rabbits were first described by Thomas (1956), who noted that the most obvious change was a bilateral collapse o f the ears. This effect was lost within a few days. Subsequently llu lth and Westerborn (1959) and Merkow and Lalieh (1961), who iLsecl crude papain in single and repeated (loses, and in dif...

Catfish (Clarias gariepinus) is a freshwater fish that widely cultivated in Indonesia. Production cost of farming activities spend 60-70% on the use feed. Therefore, feeding must be effective and efficient so catfish can make good it for its growth. The addition an exogenous enzyme form papain increase efficiency feed utilization. purpose this study was to determine effect growth survival sangk...

Currently, special attention is paid to the study of effectiveness immobilization method—microencapsulation. The aim research obtain a complex enzyme preparation from pepsin and papain by sequential microencapsulation enzymes in pseudo-boiling layer evaluate its tenderizing effect on pork. objects were enzymes: papain, which microencapsulated protective coating maltodextrin. It was found that b...

Removing sericin from the periphery of silk without damage to fibroin (SF) obtain high-molecular-weight SF is a major challenge in field SF-based biomaterials. In this study, four neutral proteases, subtilisin, trypsin, bromelain and papain, were used degum silk, degumming efficiency proteases their influence on molecular weight (MW) regenerated studied. The results indicated that all could rem...

The aim of study was to evaluate efficacy of extracted Chondroitin Sulfate (CS) from chicken keel cartilages, its comparison with standard CS from shark origin alone and in combination with Glucosamine Sulfate (GS) in developed and standardized papain induced Osteoarthritis (OA) rat model. Control group (normal) received sterile normal saline solution while experimental group’s papain intra-art...

Mulberry leaves are currently used as food for silkworms and livestock. Judging from the high protein content, it can be alternative flavoring. combined with shrimp heads which glutamic acid. Making flavoring is through a hydrolysis process using papain calotropin enzymes. The purpose to determine effect of concentration combination enzymes, well time on characteristics. This study completely r...

Various mechanisms for the reversible formation of a covalent tetrahedral complex (TC) between papain and peptidyl aldehyde inhibitors were simulated by DFT calculations, applying the quantum mechanical/self consistent reaction field (virtual solvent) [QM/SCRF(VS)] approach. Only one mechanism correlates with the experimental kinetic data. The His-Cys catalytic diad is in an N/SH protonation st...

Naive CD4(+) T cell differentiation into distinct subsets of T helper (Th) cells is a pivotal process in the initiation of the adaptive immune response. Allergens predominantly stimulate Th2 cells, causing allergic inflammation. However, why allergens induce Th2 cell differentiation is not well understood. Here we show that group 2 innate lymphoid cells (ILC2s) are required to mount a robust Th...

Leaves of 18- to 24-d-old tomato (Lycopersicon esculentum) plants exposed to gaseous methyl jasmonate (MJ) for 24 h at 30[deg]C in continuous light contained high levels of soluble protein that inhibited papain. Chromatographic analysis demonstrated that the active protein had a molecular mass of 80 to 90 kD. Induction of papain inhibitor was directly related to the concentration of air-borne M...

Studies on the degradation of animal and human serum antibody type proteins by various proteolytic enzymes have indicated that molecules of approximately one-half and one-quarter the size of the parent 160,000-molecular weight protein may be readily formed (l-5). Porter (6) has recently shown that rabbit y-globulin is degraded by papain into fragments of the above size range and has separated s...