Background: Intra-operative frozen section plays an important role in the management of surgical patients but yet it must be used prudently to avoid the indiscriminate usage of this technique. As it is subjected to many limitations in comparison to the paraffin embedded sections, this study aims to highlight the important concepts and principles of intraoperative frozen section consultation as ...

The advanced medium-throughput NanoString nCounter technology has been increasingly used for mRNA or miRNA differential expression (DE) studies due to its advantages including direct measurement of molecule expression levels without amplification, digital readout and superior applicability to formalin fixed paraffin embedded samples. However, the analysis of nCounter data is hampered because mo...

1 Division of Cariology, Operative Dentistry and Endodontics, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan. 2 Pulp Biology and Endodontics, Department of Restorative Sciences, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan 3 Department of Morphological Sciences, Biological Sciences Center, Federal University of Santa Catarina, Florianopol...

Diabetes mellitus is a progressive disease. Most investigators have focused on glomerular changes in diabetic kidney and non-glomerular alterations have been less attended. The present study has been conducted to find early non-glomerular histological changes in diabetic renal tissue. Twenty male Wistar rats weighting 200-250 g were used for the diabetic group. Diabetes mellitus was induced by ...

A series of measurements was made of the diameters of the cells and nuclei of the primary spermatocytes of the snail Helix aspersa in preparations made by embedding in paraffin wax after fixation in a number of different cytological fixatives. These were compared with similar measurements made on the living spermatocytes mounted in suitable saline solutions, and the shrinkage that the cells und...

Collagen I (Col1) fibers are a major structural component in the extracellular matrix of human breast cancers. In a preliminary pilot study, we explored the link between Col1 fiber density in primary human breast cancers and the occurrence of lymph node metastasis. Col1 fibers were detected by second harmonic generation (SHG) microscopy in primary human breast cancers from patients presenting w...

A study was undertaken to determine if it might be possible to prepare tissue sections on slides without the use of paraffin embedding, microtome sectioning, or cryostat sectioning which involve equipment and training not always available to scholars or professionals wishing to examine tissue microscopically. After evaluating many different reagents, cutting instruments and solid supports, we d...

Immunohistochemical localization of antigens in advanced third-stage larvae of Gnathostoma spinigerum (GsAL3) was studied by indirect enzyme immunostaining using 7 G. spinigerum specific monoclonal antibodies, FS-3D11, SS-5H5, SK-6C4, SK-4E1, SK-7G6, SD-8D4 and SA-9B5. All these MAb belong to the IgG1 subclass and only FS-3D11 and SS-5H5 recognize carbohydrate determinants. Each MAb exhibited a...

Introduction. Histologic changes which occur in the tunica adventitia during initiation, progression, and complications of atherosclerosis are seldom reported. This study aimed at describing the features of atherosclerosis in the tunica adventitia of two of the commonly afflicted arteries, namely, left anterior descending coronary and common carotid in black Kenyans. Materials and Methods. Spec...

The present study puts forth the morphometric analysis of modifications that occur in the ovarian structures (stroma, parenchyma, blood vessels) before and after menopause onset. Our study was performed on 30 human ovary specimens from patients with no history of genital pathology that were split in two groups (15 ovaries harvested from fertile women and 15 ovaries harvested from women at menop...