The methyl parathion hydrolase (MPH)-encoding gene mpd was placed under the control of the P43 promoter and Bacillus subtilis nprB signal peptide-encoding sequence. High-level expression and secretion of mature, authentic, and stable MPH were achieved using the protease-deficient strain B. subtilis WB800 as the host.

Brazil has experienced a rise in the use of agrochemicals recent years, representing potential threat to nearby ecosystems. The Volta Grande Reservoir (Minas Gerais/São Paulo, Brazil) about 87% its area surrounded by agricultural systems, and many compounds used agriculture end up being carried into water bodies. Given ecological risk, our purpose was assess pesticides reservoir. We collected s...

In 1994 officials from the Ohio Department of Health reported that some residents of Lorain County, Ohio, possibly had been exposed to methyl parathion (MP), a highly toxic restricted-use pesticide. The U.S. Centers for Disease Control and Prevention (CDC) assisted in the investigation by providing epidemiologic and laboratory support to the state and local health departments. Although the init...

A variety of current-use pesticides were determined in weekly composite air and rain samples collected during the 1995 and 2007 growing seasons in the Mississippi Delta (MS, USA) agricultural region. Similar sampling and analytical methods allowed for direct comparison of results. Decreased overall pesticide use in 2007 relative to 1995 generally resulted in decreased detection frequencies in a...

Pesticide residues of organophosphate (OP) and organochlorine (OC) of most commonly used classes were monitored in soil samples collected from cotton growing areas in Nawabshah district, Sindh. All the 19 soil samples presently analyzed were found contaminated with used pesticides (i.e. dichlorvos, dimethoate, methyl parathion, fenitrothion, endosulfan, mevinphos, chlorpyriphos and profenofos) ...

Two immuno-loop-mediated isothermal amplification assays (iLAMP) were developed by using a phage-borne peptide that was isolated from a cyclic eight-peptide phage library. One assay was used to screen eight organophosphorus (OP) pesticides with limits of detection (LOD) between 2 and 128 ng mL(-1). The iLAMP consisted of the competitive immuno-reaction coupled to the LAMP reaction for detection...

Pseudomonas putida JS444, isolated from p-nitrophenol (PNP) contaminated waste sites, was genetically engineered to simultaneously degrade organophosphorus pesticides (OP) and PNP. A surface anchor system derived from the ice-nucleation protein (INP) from Pseudomonas syringae was used to target the organophosphorus hydrolase (OPH) onto the surface of Pseudomonas putida JS444, reducing the poten...