We have identified a novel tRNA methyltransferase in Saccharomyces cerevisiae that we designate Trm9. This enzyme, the product of the YML014w gene, catalyzes the esterification of modified uridine nucleotides, resulting in the formation of 5-methylcarbonylmethyluridine in tRNA(Arg3) and 5-methylcarbonylmethyl-2-thiouridine in tRNA(Glu). In intact yeast cells, disruption of the TRM9 gene results...

Susceptibility testing was performed on Dientamoeba fragilis ATCC 30948 in a dixenic culture with Klebsiella pneumoniae and Bacteroides vulgatus. D. fragilis was cocultured with the bacteria in TYGM-9 medium (ATCC medium 1171). The activities of antiparasitic drugs were assessed by counting viable D. fragilis trophozoites with a hemacytometer by trypan blue exclusion. The minimal amebicidal con...

Recent years have seen a significant improvement in available treatment options for leishmaniasis. Two new drugs, miltefosine and paromomycin, have been registered for the treatment of visceral leishmaniasis (VL) in India since 2002. Combination therapy is now explored in clinical trials as a new treatment approach for VL to reduce the length of treatment and potentially prevent selection of re...

The bacterial ribosome is the primary target for many classes of antibiotics including the aminoglycosides, tetracyclins, macrolides, and oxazolidinones, all of which interact predominantly with ribosomal RNA (rRNA), thereby interfering with cellular protein synthesis. 2] These antibiotics bind selectively to RNA sites that harbor unique sequence signatures that distinguish bacterial from eukar...

The aim of the present study was to evaluate and compare efficiacy albendazole paromomycine treatments against natural Giardia spp. infection in calves. calves were divided two groups six animals each with similar parasitic loads Giardiaspp. cysts. Albendazole (20 mg/kg) paromomycin (50 administrated orally group A P, respectively, for three consecutive days. efficiacies drugs evaluated by anal...

Visceral leishmaniasis (VL) is a vector-borne parasitic disease targeting tissue macrophages. It is among the most neglected infectious diseases. Classical manifestations of VL include chronic fever, hepatosplenomegaly, and pancytopenia. Most cases can be detected through serologic and molecular testing. Although therapy has historically relied on antimonials, newer therapeutic options include ...

Background: This study aimed to evaluate the therapeutic efficacy of paromomycin sulphate on serum amyloid A (SAA) and haptoglobin (Hp) in Cryptosporidiosis goat kids. Methods: In study, stool samples were taken from kids Cryptosporidium was detected via rapid test kit. Then, by examining same microscopically, we included 9 50 mg/kg administered which spp. for five days as a treatment. For SAA ...

The 4-aminoquinoline chloroquine (CQ) is clinically used in combination with doxycycline to cure chronic Q fever, as it enhances the activity of the antibiotic against the causative bacterium Coxiella burnetii residing within macrophage phagolysosomes. As there is a similar cellular host-pathogen biology for Leishmania parasites, this study aimed to determine whether such an approach could also...