........................................................................................................................................... IX 1. GENERAL INTRODUCTION .......................................................................................................... 1 2. LITERATURE REVIEW .......................................................................................................

We report a rapid technique, based on the polymerase chain reaction (PCR), for the direct targeting, enhancement, and sequencing of previously uncharacterized cDNAs. This method is not limited to previously sequenced transcripts, since it requires only two adjacent or partially overlapping specific primers from only one side of the region to be amplified. These primers can be located anywhere w...

The ethiological factor of periodontal diseases is the presence periodontopathogens; in state imbalance with commensals they begin to affect pathologically. With a decrease number periodontopathogens biofilm, it possible restore balance and prevent or their transition stage remission. Nowadays, most informative accessible diagnostic method for determining real-time polymerase chain reaction (PC...