A rapid method is described to efficiently perform site-directed mutagenesis based on overlap extension polymerase chain reaction (OE-PCR). Two template DNA molecules in different orientations relative to only one universal primer were amplified in parallel. By choosing a high dilution of mutagenic primers it was possible to run an overlap extension PCR in only one reaction without purification...

Polymerase chain reaction (PCR) is a technique involving enzymatic amplification of nucleic acid sequences in repeated cycles of denaturation, oligonucleotide annealing and DNA polymerase extension. It is a powerful molecular biologic tool that allows the rapid production of analytic quantities of DNA from small amounts of starting material. PCR can be performed on nearly any ocular specimen or...

Introduction: Campylobacter jejuni is one of the most common causes of food poising in humans. Rapid and specific detection of these bacteria has an important role in diagnosis and treatment of infection. The aim of this study was to design a specific PCR for the detection of Campylobacter jejuni. Methods: In this experimental study, oxidoreductase gene from the Campylobacter jejuni was sele...

Background: Leptospirosis is an emerging infectious disease and is considered to be the most widespread zoonotic disease in the world. LigB is an immunogenic outer membrane protein. The leptospiral ligB gene expressed only in pathogenic Leptospira spp. The aim of this study was molecular diagnosis of pathogen Leptospires by PCR based on ligB gene. Materials and Methods: Five pathogenic Leptospi...

The current study's goal was to identify virulence factors (genes) in P. mirabilis. A total of 25/100 (25%) Proteus mirabilis were obtained from patients with otitis media and identified using culture biochemical characteristics, Polymerase chain reaction (PCR). PCR technique used detect the 16S rRNA gene all factors, including ureC gene, which encodes urease synthesis, flaA flagella fimbriae p...

Microfabricated silicon/glass-based devices with functionalities of simultaneous polymerase chain reaction (PCR) target amplification and sequence-specific electrochemical (EC) detection have been successfully developed. The microchip-based device has a reaction chamber (volume of 8 microl) formed in a silicon substrate sealed by bonding to a glass substrate. Electrode materials such as gold an...

The polymerase chain reaction (PCR) shortens conventional microbiological methods for the detection of food pathogens either by replacing the conventional biochemical and serological identification or by its direct use on pre-enrichment media or food products. PCR allows fast and highly reliable identification of bacterial taxa, particularly phenotypically atypical bacterial strains. For reliab...

UNLABELLED PREMISE OF THE STUDY Development of genetic markers can be costly and time-consuming, especially when multiple primer pairs are fluorescently labeled. This step was streamlined by combining two techniques in the same PCR reaction: (1) custom-labeling of primers by the investigator and (2) multiplexing multiple primers together in the same reaction. • METHODS AND RESULTS This tec...

Sex determination in infants and children with ambiguous genitalia usually necessitates time-consuming and costly karyotyping .We have evaluated a simple,rapid and reliable method of postnatal sex determination by amplification of X and Y specific microsatellite markers DXS6797 and SRY respectively by polymerase chain reaction(PCR). Three probands M78, M59 and M61 with ambiguous genitalia were ...

In article number 2100430 by Sung Gap Im, Kyoung G. Lee, and co-workers, an “all-in-one tube” is devised coating poly-(2-dimethylaminomethyl styrene) (pDMAMS) on a polymerase chain reaction (PCR) tube via initiated chemical vapor deposition (iCVD) to extract, amplify, detect pathogens within single tube. The all-in-one can capture the DNA of charge interaction directly perform real-time PCR, re...