To evaluate the contribution of intracellular peptidases to the growth of the 14-amino-acid (aa) auxotroph Lactobacillus helveticus CNRZ32, single- and multiple-peptidase-deletion mutants were constructed. Two broad-specificity aminopeptidases (PepC and PepN) and X-prolyl dipeptidyl aminopeptidase (PepX) were inactivated through successive cycles of chromosomal gene replacement mutagenesis. The...

neral Sanitario (PGS) en 2011 ha existido un animado debate sobre el itinerario formativo dentro del campo de la Psicología Clínica, avivado por académicos, profesionales y alumnos, pero encendido primariamente por una deficiente y caótica regulación. El punto central de la discusión es si el nuevo Máster de Psicología General Sanitaria (MPGS) es una condición imprescindible para el acceso a la...

Miscanthus has been proposed as a promising crop for phytoremediation due to its high biomass yield and remarkable adaptability to different environments. However, little is known about the resistance of Miscanthus spp. to cadmium (Cd). To determine any differences in resistance of Miscanthus to Cd, we examined plant growth, net photosynthetic rate (Pn), activities of anti-oxidant and C4 photos...

Genetic modification of crop plants to introduce desirable traits such as nutritional enhancement, disease and pest resistance, and enhanced crop productivity is increasingly seen as a promising technology for sustainable agriculture and boosting food production in the world. Independently, cultural practices that utilize alternative agriculture strategies including organic cultivation subscrib...

The aim of this work was to analyse the response of NH4 + assimilation in leaves of tobacco plants (Nicotiana tabacum L. cv. Tennessee 86), to different B applications (B1, 5 M H3BO3; B2, 10 M H3BO3; B3, 20 M H3BO3). The plants were grown under controlled environmental conditions and received a complete nutrient solution. In this experiment, we analysed the foliar concentrations of B and NH4 , ...

Abstract Background Eucalyptus is the main plantation wood species, mostly grown in aluminized acid soils. To understand response of clones to aluminum (Al) toxicity, Al-tolerant grandis × E. urophylla clone GL-9 (designated “G9”) and Al-sensitive GL-4 “W4”) were employed investigate production secretion citrate malate by roots. Results seedlings hydroponics exposed presence or absence 4.4 mM A...

Introduction Phosphorus (P) deficiency hinders cotton ( Gossypium hirustum L.) growth and development, seriously affecting lint yield fiber quality. However, it is still unclear how P fertilizer affects length. Methods Therefore, a two-year (2019-2020) pool-culture experiment was conducted using the split-plot design, with two cultivars (CCRI-79; low-P tolerant SCRC-28; sensitive) as main plot....

Regenerating maize A188 tissue cultures were examined for the presence of enzymes involved in C4 photosynthesis, for cell morphology, and for '4C labeling kinetics to study the implementation of this pathway during plant development. For comparison, sections of maize seedling leaves were examined. Protein blot analysis using antibodies to leaf enzymes showed a different profile of these enzymes...