The authors examined whether a serum pepsinogen test (SPT) based on the combination of the serum pepsinogen I level and pepsinogen I/II ratio is a good predictor of gastric cancer occurrence in a general Japanese population. A total of 2,446 subjects aged > or =40 years were classified into negative, positive, and strong-positive SPT groups and were followed prospectively for 14 years (1988-200...

A consecutive series of 51 children (mean age 11 years) who presented with recurrent abdominal pain were investigated by upper gastrointestinal endoscopy including three antral biopsies for microscopy, culture and urease testing. Serum IgG, IgA, and IgM antibodies to Campylobacter pyloni (C pylon) were measured by the ELISA technique. Serum pepsinogen I was also measured. Thirty two children sh...

ATP-binding cassette (ABC) transporters are involved in chemotherapy resistance. Multidrug-resistance protein 8 (ABCC11/MRP8) is also involved in 5-fluorouracil (5-FU) metabolism. 5-FU and its derivatives are widely used in the treatment of gastrointestinal tract cancers, but little is known about the contribution of ABCC11/MRP8 to gastrointestinal tract and related cancers. Here, we report our...

Atrophic gastritis is characterized by chronic inflammation of gastric mucosa by Helicobacter pylori infection and other factors. Helicobacter pylori infection has been linked to coronary artery disease. To our knowledge, however, no reports are available on the relationship between atrophic gastritis and coronary artery disease. In this study, we investigated the relationship between atrophic ...

OBJECTIVES In Fujian Province, China, gastric cancer is one of the leading causes of mortality among all malignant tumors. Nanjing county and Minqing county are located in inland Fujian and have similar general demographics. However, the adjusted mortality rate of gastric cancer in Minqing was found to be much higher than that in Nanjing. We sought to explore factors associated with this increa...

BACKGROUND Atrophic gastritis has been shown to be one of the long term sequelae of Helicobacter pylori infection. AIMS To determine the prevalence of atrophic gastritis in outpatients, to study the accuracy of serological methods for revealing atrophy, and to define the association of H pylori infection with atrophic gastritis in these patients. PATIENTS/METHODS A total of 207 consecutive ...

The use of molecular techniques such as quantitative RT-PCR depends on the quality of cellular RNA. In particular, RNA extraction from endoscopic biopsies is difficult with respect to yield, and especially integrity. Endoscopic biopsies taken from the gastric antrum, corpus and duodenum were subjected to various RNA extraction protocols, and the RNA was used for quantitative RT-PCR. The subsequ...

OBJECTIVE To study free- and protein-bound cobalamin absorption and the correlation with atrophic gastritis in healthy middle-aged and older subjects. DESIGN A cross-sectional study. PARTICIPANTS Fifty-two healthy subjects, aged 26 to 87 years, apparently free from conditions known to influence the cobalamin status. Middle-aged subjects were defined as those younger than 65 years of age (me...

Commercial samples of crystalline swine pepsin have been found to be heterogeneous by a number of criteria. Several active fractions can be obtained by chromatography on hydroxylapatite with phosphate buffers, pH 5.7, of increasing molarity as eluents. This has been found to be an effective procedure for evaluating the homogeneity of pepsin. End group analysis by the cyanate method yields 1 eq ...

where [E]/[E], is fractional enzyme activity, and t is reaction time. The constants C and k were determined graphically (Defares & Sneddon, 1960). The results of the effect of diethyl pyrocarbonate on DEAE-cellulose column-chromatography fractions obtained from two samples of normal gastric mucosa and three samples of stomach adenocarcinoma are presented in summary form in Table I. It will be s...